User Guide: Control Reaction for T4 DNA Ligase Activity User Guide: Self-circularization of Linear DNA User Guide: T4 DNA Ligase, 5 Weiss U, 5 x 1000 Weiss U User Guide: T4 DNA Ligase, 5 Weiss U, 200 Weiss U User Guide: DNA Insert Ligation (sticky and blunt-end) into Vector DNA...
T4 DNA Ligase催化双链DNA或RNA中毗邻的5'-磷酸基团和3'-羟基末端之间形成磷酸二酯键。酶还可以修复双链DNA、RNA或DNA/RNA复合体中的单链切口,连接DNA的粘性和平末端,但是该酶对单链核酸无酶活性。 T4 DNA Ligase需要辅因子ATP。 特点 快速–室温下10分钟内完成粘末端的连接反应。
Thermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blu...
赛默飞 15224017 T4 DNA Ligase (1 U/μL) 价格￥509 起批量≥1盒 最小起订电询 供货总量电询 发货地址浙江省杭州市 建议售价￥509/盒 更新日期2023年04月25日 即时洽谈立即询价查看联系方式 收藏产品发送留言 杭州百莱博科技有限公司 企业认证:备 ...
T4 DNA Ligase* 75-99% FastAPTM Thermosensitive Alkaline Phosphatase 99% T4 Polynucleotide Kinase 99% *T4 DNA连接酶活性需要 0.5mM ATP 通用缓冲液和直接凝胶上样 所有FastDigest快酶在FastDigest Green绿色缓冲液中都具有99%活性。此外FastDigest Green绿色缓冲液中还包含密度试剂和两种示踪染料,从而可直接将反...
T4 DNA Ligase, 5 u*/µl Component #EL0014 #EL0011 #EL0012 T4 DNA Ligase, 5 u/µl 200 u 1000 u 5x1000 u 10X T4 DNA Ligase Buffer 0.5 ml 1.5 ml 5x 1.5 ml 50% PEG Solution0.3 ml 1.5 ml 5x 1.5 ml T4 DNA Ligase LC, 1 u*/µl Component #EL0016 T4 DN...
T4DNALigase*75-100% FastAPTMThermosensitiveAlkalinePhosphatase100% T4PolynucleotideKinase100% *T4DNA连接酶活性需要0.5mMATP 通用缓冲液和直接凝胶上样 所有FastDigest快酶在FastDigestGreen绿色缓冲液中都具有100%活性。此外FastDigestGreen绿色缓冲液中还包含密度试剂和两种示踪染料,从而可直接将反应产物添加到点样孔中。
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T4 RNA Ligase and 10X Reaction Buffer should be stored at –20°C. 描述 Ambion® T4 RNA Ligase catalyzes the formation of a phosphodiester linkage between a 5'-phosphoryl-terminated nucleic acid donor and a 3'-hydroxyl-terminated nucleic acid acceptor. Substrates include RNA, DNA, and oli...
heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly...