The visualization of gamma-H2AX by can allow the assessment of DNA damage, related DNA damage proteins and DNA repair. Gamma-H2AX has applications in the detection of genomic damage caused by cytotoxic chemical agents and environmental and physical damage. 仅用于科研。不用于诊断过程。未经明确...
Gamma-H2AX 焦点形成免疫荧光检测实验步骤 1.各组细胞分别处理后,弃培养基,PBS洗涤三次(打到壁上)。 2.多聚甲醛固定20min,4°C (4%多聚甲醛)。 3.弃多聚甲醛,PBS洗涤三次,0.2%-5% (5%浓度太大,不易溶解,2%) Triton-x(覆盖爬片即可),破膜15min 4.弃5% Triton-x,PBS洗涤三次,5%BSA工作液,室温...
Replication protein A and γ-H2AX foci assembly is triggered by cellular response to DNA double-strand breaks. Exp Cell Res. 2004; 300 :320–334.Balajee, A. S., and C. R. Geard. 2004. Replication protein A and gamma- H2AX foci assembly is triggered by cellular response to DNA double-...
Gamma-H2AX is used widely as DNA damage marker in vitro, but its use for genotoxicity assessment in vivo has not been extensively investigated. Here, we developed an image analysis system for the precise quantification of the gamma-H2AX signal, which we used to monitor DNA damage in animals ...
The detection of gamma-H2AX can potentially serve as a biomarker for transformation of normal tissue to premalignant and consequently to malignant tissues. gamma-H2AX has already been investigated in a variety of cancer types, including breast, lung, colon, cervix, and ovary cancers. The prognostic...
GammaH2AX as a checkpoint main- tenance signal. Cell Cycle. 2006; 5: 1376-81. PMid:16855385. http://dx.doi.org/10.4161/cc.5.13.2899Downey M, Durocher D. γH2AX as a checkpoint maintenance signal. Cell Cycle 2006; 5:1376-1381.
Gamma-H2AX as a marker for dose deposition in the brain of wistar rats after synchrotron microbeam radiation. PLoS One. 2015;10(3):e0119924. https://doi.org/10.1371/journal.pone.0119924. Article CAS Google Scholar Fujii T, Nakamura K, Hiraga K. Effects of pH on the carcinogenicity of...
应用gammaH2AX53BP1及RAD51焦点进行DNA双链断裂损伤研究(生物学资料)目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1:应用gammaH2AX53BP1及RAD51焦点进行DNA双链断裂损伤研究 1 1、材料与方法 2 2、结果与分析 4 3、讨论 6 文2:DNA双链断裂修复蛋白在宫颈癌和CIN组织中的表达 8 1 材料与方法 8 ...
Trevigen的HT Gamma H2AX Elisa 试剂盒提供包埋有γ-H2AX抗体96孔板来捕获样本中的γ-H2AX,H2AX抗体作为检测抗体,后用山羊抗小鼠HRP标价的二抗与之结合,利用化学发光底物产生的光强度单位即可检测样本中γ-H2AX的量。该试剂盒可用于悬浮细胞、贴壁细胞人外周血单核粒细胞和组织切片等样本的检测。