The present invention relates to use of an enzyme that cleaves both alpha- and beta-1,4-glycosidic bonds and, more particularly, the present invention provides α-L-fucosidase which has α-1,4-glucosidase and β-1,4-galactosidase activities and thus is capable of producing glucose and/or ...
Many xylanases often do not cleave glycosidic bonds between xylose units that are substituted. The side chains must be cleaved before the xylan backbone can be completely hydrolyzed (Lee and Forsburg, 1987). On the other hand, several accessory enzymes only remove side chains fro...
β-glucans consist of a backbone of glucose residues linked by β-(1/3) glycosidic bonds, often with attached side-chain glucose residues joined by β (1/6) linkages. β-glucans are commonly called as biological response modifiers and they are mostly present in cereals, grains, fungi, ...
Thetertiary structureof hevamine has been explained (Terwisscha van scheltinga et al., 1994). Hevamine catalysers the cleavage of chitinβ-1,4-glycosidic bonds, the principal constituent of the parasitic cell divider, andpeptidoglycansugar moiety. Hevamine was portrayed as a resistance related bi...
Several procedures are available for the measurement of endo-1,4-β-glucanase (EG). Primary methods employ defined oligosaccharides or highly purified polysaccharides and measure the rate of hydrolysis of glycosidic bonds using a reducing-sugar method. However, these primary methods are not suitable ...
4-Methylumbelliferyl-β-D-xylopyranoside (MuX) can be used as substrate for the research of β-Xylosidase activity. 4-Methylumbelliferyl-β-D-xylopyranoside shows burst, steady-state kinetics, which supports the conclusion that hydrolysis of the glycosidic bonds for the substrate 4-Methylumbelliferyl-β...
glycosidic bond, although the bands at 930 cm−1, 1038 cm−1, 1078 cm−1, and 1161 cm−1also reflected β(1–3) glycosidic bonds. The bonds of α(1–2), and β(1–3) in l-EPSs fromL. delbrueckiissp.bulgaricusB3 andL. plantarumGD2 were compatible with the ...
The basic structure of beta-(1,3)-D-Glucan consists of glucose molecules linked together in a linear chain with beta-1,3-glycosidic bonds. This compound is insoluble in water. Beta-(1,3)-D-Glucan is generally considered safe for use, but it may cause irritation to the respiratory system...
is clearly demonstrated that the DNS method grossly overestimatesendo-xylanase activity. Unlike the DNS assay, the NS assay gave the equivalent colour response with equimolar amounts of xylose, xylobiose, xylotriose and xylotetraose demonstrating that it accurately measures the quantity of glycosidic ...
α-Glucosidase (EC. 3.2.1.20) as a membrane-bound enzyme could catalyze the hydrolysis of the glycosidic bond at the non-reducing terminal of the sugar, resulting in the release of free glucose into the digestive tract. Benzoxazole 197 (Fig. 19) having 4-phenoxy substituti...