Sample StorageEluted DNA should be stored at ≤ -20°C. Size RangeTypically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield. TypeTotal DNA YieldUp to 25 µg total DNA can be eluted into ≥50 µl ...
Total RNA Isolation (Mechanical Lysis) Total RNA Isolation (Zymolyase) Yeast Protein Purification High-Throughput/Automated Isolation ENZYMES Nucleases DNase I RNase A PureRec Duplex-Specific Nuclease PureRec RNase A Micrococcal Nuclease DNA Degradase ...
Q2: How to process naked DNA stored in DNA/RNA Shield? Use the standard protocol (add 2 or 5 volumes of DNA binding buffer depending on DNA size). Q3: What to do if ethanol addition to the DNA Wash Buffer was omitted? The DNA will be eluted off the column. Rebind samples using the...
Yes, bring samples homogenized and stored in DNA/RNA Shield to room temperature (20-30ºC). Add 3 volume of TRIzol/TRI Reagent and mix well. Proceed with RNA Purification. Q6: Is it possible to extract proteins with the Direct-zol RNA kits?
Q5: How can I process naked DNA stored in DNA/RNA Shield? Add an equal volume of ethanol (95-100%) to the sample and mix well. The sample is ready-to-bind and does not require DNA Binding Buffer. Proceed to Step 2.Q6: What is the lower limit and minimal amount of DNA that can...
Sample Collection & Preservation DNA Purification RNA Purification Total Nucleic Acid Purification NGS Library Preparation Microbiomics Epigenetics NGS Services Competent Cells & Cloning PCR & Molecular Assays Enzymes & Protein Expression Yeast Research Lab Equipment & Supplies View All Products Current Promot...
The RNA Clean & Concentrator Magbead kit provides a simple and reliable high-throughput method for the rapid preparation of high-quality, DNA-free (R1082) RNA. This simple procedure is based on the use of magnetic-bead based technology and a unique single-buffer system......
Q1: What is the difference between the Quick-RNA Miniprep and the Quick-RNA Miniprep Plus? Use the Quick-RNA Miniprep for cells and soft tissues. The “Plus” kit accommodates all sample types (cells, tissue, blood) and comes with DNA/RNA Shield (sample collection, transport, storage at ...
Use the Quick-RNA Miniprep for cells and soft tissues. The “Plus” kit accommodates all sample types (cells, tissue, blood) and comes with DNA/RNA Shield (sample collection, transport, storage at ambient temperature). Q9: I ran out of RNA Wash Buffer. Can I use something else?
Sample Collection & Preservation DNA Purification RNA Purification Total Nucleic Acid Purification NGS Library Preparation Microbiomics Epigenetics NGS Services Competent Cells & Cloning PCR & Molecular Assays Enzymes & Protein Expression Yeast Research Lab Equipment & Supplies View All Products Current Promot...