Furthermore, it was found that ENO1 was a downstream target of miR-22-3p by using bioinformatics analysis and luciferase reporter assays. MiR-22-3p downregulation promoted neuropathic pain via regulating inflammation factors expression by targeting ENO1. Then, we explored the upstream regulator of ...
In the follow-up test, circZNF609 was discovered to take a bigger proportion in cytoplasm than nucleus in HCC, indicating its post-transcriptional regulation of target gene expression. Thus, we boldly conjectured that circZNF609 might mediate the activation of Hedgehog pathway through post-...
Circular RNAs are a class of endogenous noncoding RNAs that regulate gene expression in eukaryotes. In this study, we investigated the role of cZNF609 in retinal neurodegeneration induced by glaucoma.Methods: qRT-PCR and Sanger sequencing were conducted to detect cZNF609 expression pattern during ...
Luciferase reporter assay, target gene prediction and screening were used to validate downstream target genes of circZNF609 and miR-134-5p. EGFR expression was detected by Western blot analysis and RT-qPCR. Nude mice were used to detect tumor changes. CircZNF609 was upregulated in LSCC and ...
To deepen our knowledge about circ-ZNF609 role in cell cycle regulation, we studied its expression and function in rhabdomyosarcoma (RMS), a pediatric skeletal muscle malignancy. We found that circ-ZNF609 is upregulated in biopsies from the two major RMS subtypes, embryonal (ERMS) and alveolar...
The knockdown of circ-ZNF609 in BGC823 and MGC803 cells suppressed proliferative and invasive abilities. MiRNA-145-5p was predicted to be the target gene of circ-ZNF609 by bioinformatics, and further verified by the Dual-Luciferase reporter gene assay. Rescue experiments showed that miRNA-145-...
The downstream gene and signalling of cZNF609 were studied by utilizing qRT-PCR and Western blot. Results: HaCaT cells were remarkably damaged by H2O2, as evidenced by the viability loss, apoptosis and ROS generation. It was coupled with the elevated expression of p53, p16, Bax and the ...
Q1 鈥 Q4 represents the quartile groups based on the target gene expression levels.There was no significant difference in the expression levels of circZNF609 and circPUM1 in the plasma exosomes between the IS and control groups ( P >0.05). However, a nonlinear relationship between the ...
GENE expressionHEPATOCELLULAR carcinomaVASCULAR endothelial cellsThis retraction relates to this paper Liao X, Zhan W, Tian B, Luo Y, Gu F, Li R. I OncoTargets Ther i . 2020;13:7773-7783. The Editor and Publisher of I OncoTargets and Therapy i wish to retract the published ...