此外，EMSA分析表明，O2可以特异性结合到GCN4基序，这是ZmGRAS11启动子区域的一个典型O2结合位点（图1F）。我们还通过双荧光素酶反式激活试验证明，瞬时表达的O2可以反式激活ZmGRAS11启动子，验证O2在发育中的籽粒中解释ZmGRAS11的eQTL（图1G，H）。最后，我们评估了ZmGRAS11本身是否可以作为转录激活剂。ZmGRAS11-...
We find that Opaque2, a core transcription factor for zein protein and starch accumulation, transactivates the expression of ZmGRAS11. Our data suggest that the Opaque2-ZmGRAS11 module mediates synergistic endo-sperm enlargement with grain filling.Ye LiShuai MaQianqian ZhaoDi LvBaobao WangKe Xiao...
首先,通过在泳道2中加入荧光标记的野生型ZmGRAS11探针和O2蛋白设置阳性对照,结果出现阻滞条带,表明O2蛋白可以结合野生型ZmGRAS11探针(图5B,泳道2)。其次,为证实未标记的野生型ZmGRAS11探针能与标记的探针竞争结合O2蛋白,在泳道2体系的基础...
凝胶阻滞试验(EMSA)的基本原理是:荧光标记的DNA 与蛋白质结合后分子量增大,电泳后通过荧光检测可在相应位置显示出阻滞条带。已知O2蛋白通过与ZmGRAS11基因启动子区的motif序列结合来调控 ZmGRAS11基因的表达,科学家为证实motif 序列是与O2蛋白结合的关键序列,利用荧光标记的motif序列作为指示探针,无荧光标记的motif序列...
ZmGRAS11O2ZmEXPB12Maize(Zea mays)endosperm filling is coordinated with cell expansion to enlarge the grain size,but the mechanism coupling the two processes is poorly understood.Starchy endosperm cells basically contain no visible vacuoles for cell expansion.During grain filling,efficient synthesis of ...
mays)expansionfillingMaize(Zea mays),a major crop for food and feed,is grown world-wide with a high annual yield.The grain size and weight of maize are mainly determined by starch and storage proteins ... 查看全部>>doi:10.1016/j.molp.2022.01.009Chun-Lin ShiLiuji Wu分子植物:英文版...