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Quantitative reverse transcription PCR (qRT-PCR) analysis The apical buds from RIL-46 M (csgl3), RIL-46 W (WT, CsGL3), WI7350 (csgl1) and double mutant (csgl1 + 3) were collected and flash frozen in liquid nitrogen. Total RNA was isolated with RNeasy Plant Mini Kit (Qiagen, ...
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The apical buds from RIL-46 M (csgl3), RIL-46 W (WT, CsGL3), WI7350 (csgl1) and double mutant (csgl1 + 3) were collected and flash frozen in liquid nitrogen. Total RNA was isolated with RNeasy Plant Mini Kit (Qiagen, Germany) following the manufacturer’s instructions. The ...