than 100 Xenopus finger protein-encoding cDNAs and in this study we show that at least 10 of these clones share extensive sequence homologies in a region of more than 200 amino acids in the N-terminal nonfinger portion of the predicted proteins, which is connected to variable finger clusters....
2c), suggesting that 1 mM copper or iron can induce protein precipitation of CTTNBP2 but not a conformational change. Fig. 2: Zinc directly binds and regulates CTTNBP2 phase transition. a Circular dichroism (CD) spectra of CTTNBP2104–271 in the absence or presence of zinc at the ...
ARTICLE Received 1 Jun 2015 | Accepted 16 Nov 2015 | Published 16 Dec 2015 DOI: 10.1038/ncomms10195 OPEN Epigenetic regulation of puberty via Zinc finger protein-mediated transcriptional repression Alejandro Lomniczi1,*, Hollis Wright1,*, Juan Manuel Castellano1,2,*,w, Valerie Matagne1,*,w...
Based on these features, we have named the protein Cdc48-associated UBL/zinc finger protein-1 (Cuz1). Cuz1 is highly conserved, with the most similar human protein being the uncharacterized ZFAND1 polypeptide. Cuz1 binds directly to Cdc48 and also to the proteasome; the latter interaction ...
The poly(A)-binding protein (PABP), a key component of different ribonucleoprotein complexes, plays a crucial role in the control of mRNA translation rates, stability, and subcellular targeting. In this study we identify RING zinc finger protein Makorin 1 (MKRN1), a bona fide RNA-binding ...
et al. Regulation of apoptosis and cell cycle arrest by Zac1, a novel zinc finger protein expressed in the pituitary gland and the brain. The EMBO journal 16, 2814–2825 (1997). 44. Stocco, D. M. StAR protein and the regulation of steroid hormone biosynthesis. Annual Review of ...
We hypothesized that this novel KRAB-containing zinc finger protein has a distinct and essential role in the development of the early bovine embryos. Herein, we report the identification and cloning of cDNA encoding ZNFO, the temporal expression of ZNFO mRNA during oocyte maturation and early ...
Since in Drosophila the actions of Hh have been shown to be transduced by Cubitus interruptus (Ci), a zinc finger transcription factor, we examined whether a vertebrate homolog of this protein can mediate the functions of Shh in the vertebrate nervous system. Here, we demonstrate that ...
The Zinc Finger Cluster Domain of RanBP2 Associates Specifically with a 110-kDa Retinal Protein We continued the structure-function analysis of RanBP2 by focusing this time on the zinc finger cluster domain (ZnF) of RanBP2 (Fig. 1 a). We used the bovine five zinc finger motifs of RanBP2...
APLP1 E2 antiserum was purified on a HiTrap Protein A HP column (GE Healthcare). The antisera displayed no cross-reactivity with other APP family proteins. Immunodetection was performed with anti-APLP1 E2 (1:2000) or anti-APLP2 E2 (1:500), followed by incubation with HRP-coupled anti-...