Additionally, the effect of YTHDC1 knockdown on CD86 and IL-6 mRNA induced by LPS/IFN-γ was abolished by the NF-κB inhibitor JSH-23. Mechanistically, YTHDC1 interacted with Beclin1 mRNA, thereby stabilizing Beclin1 mRNA and enhancing Beclin1 expression and autophagy. These effects ultimately...
Gene expression profiling of MOLM13 tumors also demonstrate inhibition of MYC target gene sets among the most significant changes upon dosing with YTHDC1 inhibitor. Overall, our data suggests that pharmacological inhibition of YTHDC1 condensates utilizing our optimized small molecule inhibitors presents ...
Recombinant YTHDC1 (325-502) protein is suitable for use in the study of binding assay, inhibitor screening, and selectivity profiling Binding Assay Conditions: 3 uM oligo m6A ssDNA (GTTGG/m6A/CTT) was incubated with different concentrations of YTHDC1 (325-502) protein in 10 ul reaction sys...
HK1, SLC2A1 was measured by western blot after transfection with miR-30d inhibitor and subsequent knockdown of RUNX1.h–lCell proliferation measured by CCK8 assay, colony formation assay, cell migration by transwell assay, cell invasion by transwell assay, tumor angiogenesis by ...
5C, D). In addition, reducing PTEN expression in shYTHDC1-transfected neurons by treating cells with PTEN inhibitor bpv37,38 restored the phosphorylation of Akt (Fig. 5E, F). Therefore, YTHDC1 may promote neuronal survival especially after OGD through enhancing PTEN expression. YTHDC1 ...
The KEGG enrichment analysis of the RNA-seq of YTHDC1 showed that YTHDC1 was involved in the EGFR tyrosine kinase inhibitor resistance pathways in A498 cells (Fig. 3B). We previously reported that ANXA1 participates in modulating the sensitivity to sunitinib in ccRCC [17]. The literature ...
5D–I). qRT-PCR, western blot, and immunofluorescence assays showed that the miR-124486-5 mimic decreased the MAPK1 mRNA, t-ERK1/2 and p-ERK1/2 proteins expression, and the p-ERK1/2 level against the amount of t-ERK1/2 expression, whereas the miR-124486-5 inhibitor increased it (...
1. Expression profiles of miR-451a and overexpression/knockdown efficiency of miR-451a mimics/inhibitor. (A) Volcano plot of differently expressed miRNAs in osteosarcoma (tumor) and normal samples on the GEO datasets (GSE28423). Red dots presented upregulated miRNAs in tumor samples relative to ...
Mechanistically, YTHDC1 interacts with nuclear factor kappa B (NF-魏B) inhibitor-alpha mRNA, suggesting its involvement in BMSC survival pathways. This study identifies YTHDC1 as a potential target to enhance BMSC efficacy in MI therapy.
Serine protease inhibitor A3N-siRNA reduced LPS-induced inflammation of cardiac myocytes. In conclusion, the m(6)A reader YTHDC1 regulates SERPINA3N mRNA expression to mediate the levels of inflammation in SIC. Such findings add to the relationship between m(6)A reader YTHDC1 and SIC, ...