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Method details 方法细节 Plasmids and shRNAs 质粒和shRNA Para_01 TRC慢病毒载体编码针对YTHDF2、PABPC1、ATP5PB、ATP5MG、ATP5MF、NSUN2和METTL3的shRNA是从GE Dharmacon(科罗拉多州拉斐特)或MilliporeSigma购买的。 YTHDF2、ATP5PB、ATP5MF、ATP5MG和PABPC1的CDS是从B-ALL细胞系cDNA通过PCR扩增获得的。
use the method you mentioned, I could yt-dlp most of the clips which I want in bbc. dirkf commented on Sep 30, 2024 dirkf on Sep 30, 2024 Contributor I've now had time to check the quoted excerpt, which I believe was the only part referencing the problem video. Confusingly, it...
β-actin was used as an internal reference, and RNA expression was analyzed using the 2−ΔΔCT method. Primer sequences were synthesized by BGI (Beijing, China) and are listed in Supplementary Material 8: Table S2. Wound healing assays Cells were seeded in a six-well plate and incubated...
Disk diffusion methodControl: kanamycin EtOH extract exhibited the highest activity amongst all tested extractsAll the tested samples exhibited antibacterial activity Khan et al. (2001) P. careya H2O and MeOH ext. of leaf, 20, 79, 80, 113, 117, 121, 135, and 136 B. cereus, S. aureus,...
The grouping method was the same as that in the section describing the evaluation of somatic signs. On days 1 and 2, all the mice had free access to the entire apparatus for 15 min to reduce the effects caused by the experimental environment. Animals with a strong initial preference for ...
1. An immunoblot methodfor the quantitative screening of a large number of reactivities.Scand J Immunol 1994;39:7949.Lacroix-Desmazes S, Resnic kI, Stahl D, Mouthon L, Espanol T,Levy J, Kaveri SV, Notarangelo L, Eibl M, Fischer A, Ochs H, Ka-zatchkine MD. Defective self-reactive ...
Steroid quantification and method validation Stock solutions (1 mg/mL) of each authentic standard 17β-Estradiol (E2), Estrone (E1), Cortisol and Cortisone were prepared in MeOH and were further diluted in H2O/MeOH (1:1) to prepare a mixture of standards of suitable concentrations. IS we...
****p < 1 · 10−4, n.s., not significantly different, using Mann-Whitney U test. n(control) = 27, n(LNX2) = 27. Full size image Ablating LNX2 in neurons increases the expression of endogenous GlyT2 We next decided to explore whether neuronal GlyT2 is ...
To identify AS event, PSI method applied previously was used (Melé et al., 2015). Briefly, RNA-seq reads were mapped to the EEJ (exon-exon junction) libraries with BWA (Li and Durbin, 2009), and PSI (percentage spliced in) level of the exon was quantified. The PAR-CLIP-seq data ...