Synthetic promoters, especially minimally sized, are critical for advancing fungal synthetic biology. Fungal promoters often span hundreds of base pairs, nearly ten times the amount of bacterial counterparts. This size limits large-scale synthetic biolog
Yeast minimal media/synthetic-defined (SD) premixes Get conveniently premixed/synthetic defined (SD) minimal yeast media; choice of broths or agar. No weighing, mixing, or pH adjustment needed. Ready-to-go media pouches for yeast culture ...
association of Mmm1-ERFP with Dnm1-GFP patches. Bar, 5 μm. (D) Strains were transformed with a multicopy plasmid for overexpression ofMDM33from the inducibleGAL1/10promoter (pYX223-MDM33) or an empty vector. 10-fold serial dilutions were spotted on synthetic complete medium containing ...
PROBLEM TO BE SOLVED: To provide a semisynthetic medium and a synthetic medium to solve problems caused by the use of blackstrap molasses in the conventional baker's yeast production process and to develop a culture method to use the medium.;SOLUTION: An improved synthetic medium containing an ...
We develop a method termed SCRaMbLE-based genome compaction (SGC) and demonstrate that a synthetic chromosome arm (synXIIL) can be efficiently reduced. The pre-introduced episomal essential gene array significantly enhances the compacting ability of SGC, not only by enabling the deletion of nonessent...
Yeast were grown in synthetic minimal media containing yeast nitrogen base (YNB), 2% glucose and supplemented with the required amino acids (aa) or bases. When expression of the GAL1 promoter was required, the glucose was substituted with 2% galactose and 2% raffinose. Yeast cells were transform...
curcas, regulated by the galactose-inducible GAL1 promoter, were transformed into wild-type yeast strain BY4741; following heat-shock at 15 min at 42°C, the yeast cells were revived in YPD media and plated on synthetic minimal medium plates lacking uracil (see additional file 4: stock ...
关于产品“酵母营养缺陷型培养基 Drop-out Mix Complete w/o Yeast Nitrogen Base (Powder)”的详情 Name:Drop-out Mix Complete w/o Yeast Nitrogen Base (DO, Dropout, Powder)Desc:Drop-out Mixes are used as a supplement to Drop-out Base for making synthetic, complete media formulations in accordanc...
Addition of pancreatic lipase to wild-type LDs stimulated the lipolytic activity ∼ 30-fold and led to almost complete degradation of TAG into glycerol and FFA. In contrast, pancreatic lipase caused a massive accumulation of MAG in Yju3p-deficient preparations (Fig. 4C). Download: Download ...
Name:Drop-out Mix Synthetic, Minus Adenine and Histidine w/o Yeast Nitrogen BaseDesc:Drop-out Mix Complete (DOC) is used as a supplement to Drop-out Base for making synthetic, complete media formulations in accordance with Methods in Yeast Genetics.All lots are quality control tested using the...