(A) Flow of genetic information (central dogma of molecular biology) from DNA to RNA to proteins [56–58] (solid arrows), and whole spectrum of biological interactions/networks at all different levels (from supramolecular to molecular level) in the eukaryotic cell: DNA–DNA; DNA–RNA; DNA–...
There is no doubt that DNA-metabarcoding can be adapted to document the extensive and vast biodiversity of yeasts, but for practical reasons, such as clinical studies and biotechnological applications, it is essential to obtain, and study isolates themselves. Additionally, for the identification, DNA...
Sex is the prevailing reproductive mode throughout the eukaryotic tree of life [1]. At its core lies a periodic ploidy cycling, accomplished through meiosis, during which haploid gametes are produced, and mating, which ensures the restoration of the original ploidy level. Meiosis, thought to have...
While a commonly-used S288c-derived lab strain of yeast is unable to acquire further H2O2resistance following mild ethanol pretreatment, most wild yeast strains can [50]. Experiments designed to identify the mechanistic basis of this difference led to the discovery that cytosolic catalase T (Ctt1...
Bead lysis is a novel method for disrupting yeast cells, studies have shown that this method helped to improve the adsorption of metal ions to the cells (Gohari, Hosseini, Sharifnia, & Khatami, 2013). Overall, the mechanical methods are simple and practical for destroying yeast cells, but ...
a “positive control.” Surprisingly, YPS163ctt1∆ mutants retained substantial acquired H2O2resistance following mild salt pretreatment (~ 25% of wild-type resistance or an additional 1 mM of resistance compared to the mock-treated control, Fig.1), a level of resistance expected to be ...
Furthermore, it has been reported that, beyond the cryoprotection, an increased level of intracellular glycerol has several benefits for the shelf life of wet yeast products and for the leavening activity (Myers et al., 1998; Hirasawa & Yokoigawa 2001; Izawa et al., 2004a) and no effect ...
A practical recipe for stable isotope labeling by amino acids in cell culture (SILAC). Nat. Protoc. 1, 2650–2660 (2006). 19. Caspi, R. et al. The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of Pathway/Genome Databases. Nucleic Acids Res. 42, D459–...
- 《Bmc Plant Biology》 被引量: 57发表: 2013年 AhR/Arnt:XRE interaction: turning false negatives into true positives in the modified yeast one-hybrid assay. Given the frequent occurrence of false negatives in yeast genetic assays, it is both interesting and practical to address the possible ...
reflect different RNA molecules that are independently translated or a competition occurring at the translation level between overlapping ORFs. Nonetheless, our results suggest that despite their low fractions of in-frame reads, these ORFs might be associated with a translation outcome and, therefore, ...