aPhotograph of FEY_2 streaked onto an agar plate, showing a functional carotenoid pathway. The illustration shows the engineering signatures comprising FEY_2, which included a carotenoid pathway chromosomal integration, a low copy plasmid expressing dCas9, and a high copy plasmid expressing gRNA.bApp...
The potential use of yeasts to control grain-storage molds was evaluated by coculturing the yeast with and on agar plates, using different temperatures, water activities (), and nutrient concentrations. Addition of 10 ppm cycloheximide to malt-extract agar inhibited completely without affecting ...
RLS is classically assayed by micro-dissecting yeast cells on a nutrient-rich agar plate under a microscope; typically, more than 20 “virgin” or undivided cells are separated for each strain to be tested, then these cells are allowed to divide. After division, daughter cells are separated ...
Product Name:Yeast leaching powdered glucose agar (YPD) plate Synonyms: CAS: MF: MW:0 EINECS: Mol File:Mol File Browse by Nationality Suppliers>Global suppliers Beijing (3)Shanghai (5)Guangdong (1)Member(9)All (9) Please select the suppliers ...
starkeyi cells was inoculated from a YM-agar plate into 100 ml YPD-medium in 500 ml baffled shake flasks and incubated at 25 °C and 150 rpm in a rotatory shaker. After 48 h, the 100 ml culture was transferred to 400 ml YPD medium in a 3 l shake flask and ...
5. Restreak the colonies onto a fresh plate every week. Stock Preservation Yeast strains can be stored indefinitely in 15% (v/v) glycerol at ≤–60°C. (Yeast cannot be stored at temperatures above –55°C for prolonged periods.) Grow the strains on YPAD agar plates. Scrape the yeast...
about 2.5 OD600(which constitutes about 2.3 mg of wet weight) ofyeast cells were harvested by centrifugation fromliquid culture or scraped off the agar plate using abacteriological loop. These cells were resuspendedin 100 ml distilled water, added 100 ml0.2 MNaOH,incubated for 5 min at room te...
A fungal contaminant on an agar plate containing colonies of Xanthophyllomyces dendrorhous markedly increased carotenoid production by yeast colonies near ... EE Carlos,EA Johnson - 《Fems Yeast Research》 被引量: 39发表: 2010年 加载更多来源...
The five-plate TF-pair yeast array and DNA-baits were mated pairwise on permissive media agar plates and incubated at 30 °C for 1 day. Mated yeast were then transferred to selective media agar plates lacking uracil, leucine, and tryptophan to select for successfully mated yeast and ...
aSpot assays for nanobody and nanobody-mKate2 expression. Strain construction is indicated at the top of the figure, along with assay temperature. Pictures were taken after 48 h on 2% galactose containing agar media. Mock specifies mKate2 expression alone.bFluorescence images of cells expressi...