Yeast display is highly amenable to directed evolution approaches, where iterative cycles of mutation and selection can be used to evolve proteins with enhanced properties, such as increased stability, binding affinity, or enzymatic activity.
摘要: In yeast surface display, yeast cells are exploited to express a protein of interest on their surface, thereby linking it to the encoding DNA within the cell. This display system has become a widely uDOI: 10.1007/978-3-642-01144-3_15 被引量: 5 ...
National, Pacific Northwest
Yeast Display Systems搜索 (21)(54) YEAST DISPLAY SYSTEMS(71) Applicant: Andreas Loew, Somerville, MA (US) (’1(72) Liventor: Andreas Loew, Somerville, MA (US)(73) Assignee: Novartis AG, Basel (CH)Appl. No.: 15/135,438(22) Filed: Apr. 21, 2016Related U.S. Application Data(63)...
Phage and Yeast Display 来自 学术范 喜欢 0 阅读量: 27 作者:J Sheehan,WA Marasco 关键词: antibody drug discovery infectious diseases phage display technology therapeutic antibodies vaccine development yeast display technology DOI: 10.1128/9781555817411.ch6 年份: 2015 ...
酵母细胞表面展示技术(Yeast surface display,YSD)是一种真核蛋白表达系统。其原理是将外源靶蛋白基因与特定的载体基因序列融合后导入酵母细胞,利用酵母细胞内蛋白转运机制将靶蛋白表达并定位在酵母细胞表面。 酵母表面展示技术在蛋白质工程、cDNA文库构建、抗体文库构建与抗体工程、生物测定和工业等多个领域得到应用。例如...
Get shareable link Copy to clipboard Keywords yeast display MHC ligands T cell epitopes RIPPA SARS-CoV-2 S Subjects Antigen presentation MHC class II Cellular & Molecular Immunology (Cell Mol Immunol)ISSN2042-0226(online)ISSN1672-7681(print)...
Fusions to the C-terminal end of the Aga2p mating adhesion of Saccharomyces cerevisiae have been used in many studies for the selection of affinity reagents by yeast display followed by flow cytometric analysis. Here we present an improved yeast display
【摘要】:正酵母表面展示(Yeast surface display)是将特定肽或蛋白质基因与酵母自身分泌到细胞外或定位在细胞壁上的基因 … cpfd.cnki.com.cn|基于4个网页 2. 酵母表面展示技术 ...ll surface 37 display) 、酵母表面展示技术(Yeast surface display) 等〔 ～39〕。
To solve this problem, we report a fullyin vitroplatform for nanobody discovery based on yeast surface display. We provide a blueprint for identifying nanobodies, demonstrate the utility of the library by crystallizing a nanobody with its antigen, and most importantly, we utilize the platform ...