1、酵母化学感受态细胞制备试剂盒(Yeast Chemical Competent Cell Preparation Ki)载体酶切:将表达质粒用限制性内切酶(同引物的酶切位点)进行双酶切,酶切产物行琼脂糖电泳后,用胶回收Kit或冻融法回收载体大片段。 2、PCR产物双酶切后回收,在T4DNA连接酶作用下连接入载体。
We also describe a protocol for the preparation of transformation-competent cells that can be stored at −70°C. The frozen cells are transformed with plasmid DNA in the presence of single-stranded carrier DNA and PEG. The numbers ... RH Schiestl,P Manivasakam,RA Woods,... - 《Methods...
Fission yeast chemical competent cell preparation kit (including transformation solution) 20 times fee information, including chemical properties, structure, melting point, boiling point, density, formula, molecular weight, uses, prices, suppliers, SDS a
et al. Condensed protocol for competent cell preparation and transformation of the methylotrophic yeast Pichia pastoris. BioTechniques 38, 44 (2005). 46, 48. Article PubMed PubMed Central CAS Google Scholar de Lima, P. B. A. et al. Novel homologous lactate transporter improves l-lactic ...
About this protocol Cite this protocol Dunn, E.A., Rader, S.D. (2014). Preparation of Yeast Whole Cell Splicing Extract. In: Hertel, K. (eds) Spliceosomal Pre-mRNA Splicing. Methods in Molecular Biology, vol 1126. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-980-...
Yeastmaker Yeast transformation system 2 PT1172-1[1]
ChemicalBook provide Chemical industry users with Fission yeast chemically competent cell preparation kit() Boiling point Melting point,Fission yeast chemically competent cell preparation kit() Density MSDS Formula Use,If You also need to Fission yeast c
Condensed protocol for competent cell preparation and transformation of the methylotrophic yeast Pichia pastoris. Biotechniques. 2005;38:44–8. CAS PubMed PubMed Central Google Scholar Yang J, Nie L, Chen B, Liu Y, Kong Y, Wang H, Diao L. Hygromycin-resistance vectors for gene expression...
Spheroplast transformation requires the removal of the yeast cell wall with β-glucanase or β-glucuronidase. The spheroplasts are incubated with plasmid DNA and PEG and plated in osmotically stabilized agar. We also describe a protocol for the preparation of transformation-competent cells that can ...
plasmids from FEY_2 using a yeast miniprep kit, so we reasoned that the sequencing library preparation step was so gentle that it was not linearizing circular plasmids for adapter ligation. Thus, we turned to a tagmentation library preparation method, the ONT Rapid kit. The improvement in ...