Protocol: Testing Your Bait for Toxicity 16 VIII. Two-Hybrid Library Screening Using Yeast Mating 17 IX.Analysis of Results 21 A. Too Few Positives 21 B. Too Many Positives 21 X. Confirmation of Positive Interactions Rescue of the Prey Plasmid 22 A. Protocol: Yeast Colony PCR Analysis to ...
Yeast Colony PCR Buffer 6-3739 10mL 995英文名称:Yeast Colony PCR Buffer 运输:4-25℃ 保存:4-25℃ 有效期:1 Year 货期:2-4 days 其他: 产品介绍:收起 Order Online在线订购 订货信息 产品名称 * 申请类型 * 套数 * 推荐人 真实姓名 * 邮寄地址 * 所属单位 电子邮箱 * 联系电话 * 联系QQ 留...
optimized protocol for isolating plasmids from yeast • PCR amplification and yeast colony hybridization protocols for the rapid analysis of positive clones obtained in a library screening • a small-scale, lithium acetate yeast transformation protocol • additional protocols for working with certain ...
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中文名称:酵母菌落直接PCR检测试剂盒 中文同义词:酵母菌落直接PCR检测试剂盒 英文名称:Yeast Colony Direct PCR Detection Kit 英文同义词: CAS号: 分子式: 分子量:0 EINECS号: 信息错误报告 您的Email: 按国家浏览Yeast Colony Direct PCR Detection Kit国外供应商中国供应商 ...
Colony PCR DNA Sequencing Sterilization Reagents Antibodies Protein Experimental Models Saccharomyces cerevisiae Kits Other Keywords Protein–protein interaction Bait protein cDNA libraries Liquid screening Fields S, Song O (1989) A novel genetic system to detect protein-protein interactions. Nature 340:245–...
Insertion was confirmed by colony PCR. A correct clone was restreaked both on YPD with 1 µM estradiol, and loss of the Cas9 plasmid was confirmed by spotting colonies onto YPD with 1 µM estradiol and YPD with 1 µM estradiol and 100 µg/ml nourseothricin. Strains with ...
α-GalQuantitativeAssay32VII.WorkingwithYeastPlasmids34A.GeneralInformation34B.PlasmidIsolationFromYeast34C.TransformingE.coliwithYeastPlasmids36VIII.AnalysisofYeastPlasmidInsertsbyPCR39A.GeneralInformation39B.TipsforSuccessfulPCRofYeastPlasmidTemplates39IX.AdditionalUsefulProtocols42A.YeastColonyHybridization42B....
What lyticase is recommended for preparation of Pichia for the PCR protocol? Can zymolyase be substituted? The lyticase for our protocol is crude lyticase. A pure preparation is more expensive and it is not necessary. We routinely ...
(1992). Chromosomal assignment of human YAC clones by fluorescence in situ hybridization: Use of single-yeast-colony PCR and multiple labeling. Genomics 14: 181- 184.Baldini A, Ross M, Nizetic D, Vatcheva R, Lindsay EA, Lehrach H, Siniscalco M (1992) Chromosomal assignment of human YAC ...