在Y2HGold酵母菌株中,只有当两个蛋白之间相互作用并结合到Gal4反应性启动子上才能活化报告基因(AUR1-C, ADE2, HIS3, 和MEL1)的表达。 酵母双杂交系统重要元件介绍(以Matchmarker GAL4-based two hybrid assay为例) 一、诱饵(the bait) 为了建立GAL4 DNA-BD/bait融合蛋白,推荐使用质粒pGBKT7; 要调查三元...
百度试题 题目酵母双杂交(yeast two-hybrid) 相关知识点: 试题来源: 解析 利用杂交基因通过激活报道基因的表达探测蛋白质与蛋白质间的相互作用。
Definition Yeast 2-hybrid is a technique used to identify proteinprotein interactions, based on the idea that if two proteins interact a reporter gene is activated. This approach is often used to identify unknown interactors (prey) of a known protein (bait). The yeast GAL-4 gene is split ...
Batch processing of yeast 2-hybrid screens allows for direct comparison of the interaction profiles of multiple bait proteins with a highly complex set of prey fusion proteins. Here, we describe refined methods, new reagents, and how to implement their use for such screens. Abstract Screening for...
A yeast two-hybrid smart-pool-array system for protein-interaction mapping. Nat Methods, 2007, 4(5): 405-7Jin, F. L., Avramova, L., Huang, J., and Hazbun, T. (2007) A yeast two-hybrid smart-pool-array system for protein-interaction mapping. Nature Methods 4 , 405–407....
Using the yeast two-hybrid system, we have isolated a cDNA (designated BBP, for Bcl2-binding protein) for a protein (Bbp) that interacts with Bcl2. Bbp is identical to 53BP2, a partial clone of which was previously isolated in a two-hybr... L Naumovski,ML Cleary - 《Molecular & ...
To investigate the architecture of the FA protein complex, the yeast 2-hybrid system was used to map contact points of the FANCA/FANCG, FANCC/FANCE, and FANCF/FANCG interactions. FANCG was shown to interact with both the amino-terminus of FANCA and the carboxyl-terminal region of FANCF. ...
To identify the co-factors that may interact with Runx2 together to regulate this critical process, we have performed yeast two-hybrid (Y2H) screen- ing using Runx2 as a bait to screen a cDNA library of hypertrophic chondrocytes. The bait expressing cassette was constructed by fusing Runx2 ...
the amplified cDNA library was 1.7×10~7 cfu/mL,the inserted fragment size of recombinants was 0.5-3 kb and the percentage of recombinant clones was 80%.Results indicate that the cDNA library is desirable which can be directly used for the yeast two-hybrid system to screen interacting ...
Identification of BCL-2 as an interaction partner for BEX1 by a yeast two-hybrid screen.Qian XiaoYeting HuYue LiuZhanhuai WangHaitao GengLifeng HuDengyong XuKe WangLei ZhengShu Zheng