However, the function of m5C modification in the nervous system is still largely unknown. Here, we show that the m5C reader Ybx1 is highly expressed in the developing mouse hippocampus in the central nervous system (CNS). Conditional knockout of Ybx1 in the dentate gyrus (DG) decreases ...
However, the roles of the m5C modification and its interaction with host proteins in regulating HCV's life cycle, remain unexplored. Here, we demonstrate that HCV infection enhances the expression of the host m5C reader YBX1 through the transcription factor MAX. YBX1 acts as an m5C reader, ...
The RNA binding protein YBX1 functions as the m5C reader and is involved in NSUN2-mediated E2F1 regulation. m5C modification promotes YBX1 phase separation, which upregulates E2F1 expression. In ovarian cancer, NSUN2 and YBX1 are amplified and upregulated, and higher expression of NSUN2 and...
Jeffrey B. KoppNational Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD .Zixuan FengShengjing Hospital of China Medical University, Shenyang, China;Feifei MiaoShengjing Hospital of China Medical University, Shenyang, China;Congcong Jiao...
NSUN2 methylated QSOX1 coding sequence region, leading to enhanced QSOX1 translation through m5C reader Y-box binding protein 1 (YBX1).#Our study reveals a critical function of aberrant RNA m5C modification via the NSUN2-YBX1-QSOX1 axis in mediating intrinsic resistance to gefitinib in EGFR-...
[60], and its role in modulating circRNA stability in collaboration with the m6A reader protein YTHDC1 [61]. As previously reported by other studies, YBX1 is recognized as a dedicated m5C reader protein [62]. Thus, the potential role of the YBX1-DDX5 complex in discerning m5C-modified ...
Secondly, YBX1 (role as RNA m5C and m6A reader protein and chemotherapy resistance gene) was screened out by three database (RBP2GO, HiPredict and GEPIA) followed with in vivo CO-IP experiment and immunofluorescence colocalization. The YBX1 expression level was not affected by PPM1G from ...
As a crucial m5C reader protein, YBX1 can bind to m5C sites on mRNA through two tryptophan residues (Trp45 and Trp65) in its CSD domain. It specifically recognizes mRNA transcripts modified by 5-methylcytosine (m5C) and promotes mRNA stability in an m5C-dependent manner by recruiting the mRN...
Different from the past focus on the nuclear function of YBX1, our works explained its role as an m5C reader stabilizing mRNA in the cisplatin resistant ovarian cancer, without addressing the distribution inside or outside the nucleus. In our study, we found that high levels of YBX1 ...
36, 37 However, the YBX1-W65A mutant successfully rescued the phenotypes of YBX1-deficient leukemia cells, suggesting that the function of YBX1 as an m5C reader is not required for its effect on myeloid leukemia. Meanwhile, YBX1-W65A, but not ΔCSD, restored the expression of BCL2 and...