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5′- GCAAAGAGCCATCTGCGACA -3′ (reverse). Total RNA of cells was extracted using TRIzol reagent (Invitrogen, USA). Then, cDNA was obtained by reverse transcription using a Vazyme HiScript III RT
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validated by quantitative polymerase chain reaction (Q-PCR) using human YBX1 primers (forward: 5’-AAGTGATGGAGGGTGCTGAC-3’ and reverse: 5’- TTCTTCATTGCCGTCCTCTC-3’) and housekeeping GAPDH primers (forward:5’- ACCCAGAAGACTGTGGATGG −3’ and reverse: 5’- CAGTGAGCTTCCCGTTCAG −3...
(Supplementary Fig.1f, g). Grain quality analysis showed that the mutation of eitherNCED2orDG1significantly increased rice AC and decreased GC (Fig.1band Supplementary Fig.1h), which was consistent with the results of fluridone treatment. Therefore, both pharmacological experiments and rice mutant ...
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