The blots were incubated with YAP1 (Proteintech, 13584-1-AP, 1:750), Slug (Cell Signaling, #9585, 1:300), E-cadherin (Cell Signaling, #9562, 1:1000), vimentin (Cell Signaling, #5741, 1: 1000), Zo-1 (Proteintech, 21773-1-AP, 1:500), and GAPDH (ABclonal, AC002, 1:1000) ...
The tissues were homogenised through ultrasonication (30 Hz, 2 min), placed on ice for 1 h, and centrifuged at 4 °C, 12,000 rpm for 10 min. The concentration of lipopolysaccharide (LPS) in the liver tissue was determined using an ELISA kit (Abclonal, Shanghai, China). The protein con...
The concentration of lipopolysaccharide (LPS) in the liver tissue was determined using an ELISA kit (Abclonal, Shanghai, China). The protein concentration was measured using a BCA kit (Yeason, Shanghai, China). D-lactate was used as an indicator of intestinal barrier permeability [38]. D-...
The antibodies to GPX4 (A1933), GAPDH (AC001), p-NPM1 Thr199 (AP0836), Bax (A19684), Bcl2 (A0208), and YAP1 (A1002) were purchased from ABclonal. The antibody to AURKA (#14475) was purchased from Cell Signaling Technology. The antibodies to p-NPM1 Ser125 (AF3740), p-NPM1 ...
After blocking, the membranes were incubated with primary antibodies against USP15 (1:200, sc515688, Santa Cruz), YAP1 (1:1000, 13584-1-AP, Proteintech), TAZ (1:1000, 23306-1-AP, Proteintech), and PCNA (1:1000, A12427, Abclonal) at 4 °C overnight. Histone H3 (1:500, 17168...
The other antibodies include Phospho-LATS1/2 (Ser909/872) (AP0904, Abclonal), N-cadherin (#33-3900, ZYMED). Immunohistochemistry (IHC) and immunofluorescence (IF) IHC was conducted on the TMA and xenograft samples using Ventana NexES automated Stainer (Ventana Corporation). Primary antibodies ...
(CST, #5023, 1:1000), anti-Bcl-2 (CST, #4223, 1:1000), anti-E-cadherin (ABclonal, A20798, 1:1000), anti-N-cadherin (ABclonal, A19083, 1:1000), anti-ZEB1 (Proteintech, 21,544–1-AP, 1:2000), anti-ATG16L1 (CST, #8089, 1:1000), anti-Beclin-1 (CST, #3495, 1:1000)...