Highly specific and rigorously validated in-house, YAP (D8H1X) XP® Rabbit Monoclonal Antibody (CST #14074) is ready to ship. 下单购买 下单前请务必联系店铺客服,提供全程优质服务。 查询产品访问优宁维商城:https://www.univ-bio.com/,
This Cell Signaling Technology antibody is conjugated to Alexa Fluor®488 fluorescent dye and tested in-house for direct flow cytometric analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated YAP ...
This Cell Signaling Technology antibody is conjugated to Alexa Fluor®647 fluorescent dye and tested in-house for direct flow cytometric analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated YAP (D8H1X) XP®Rabbit...
Anti-YAP, anti-GDF15, anti-EZH2 and anti-G9a antibodies were purchased from Cell Signaling Technology (#14074, #3249, #5246, #3306, Beverly, MA, USA). Anti-TEAD4 antibody was purchased from Sigma-Aldrich (WH0007004M1). Anti-E-cadherin and anti-N-Cadherin antibodies were purchased from ...
We used radioimmunoprecipitation assay buffer to extract protein from SMCs. Twenty micrograms of protein were loaded in 6% sodium dodecyl sulfate–polyacrylamide gel electrophoresis gel. Antibodies used were as follows: anti‐Sp‐...
Primary antibodies against YAP (#14074), androgen receptor (AR) (#5153), pan-TEAD (#13295) and histone (#7631) were purchased from Cell Signaling Technology (Beverly, MA, USA). DHT (dihydrotestosterone) and MDV3100 (enzalutamide) were purchased from Sigma-Aldrich (St. Louis, MO, USA). ...
The supernatants were incubated with anti-YAP antibody (#14074 CST) for 24 h at 4 °C, then incubated with protein A agarose beads for 1 h at 4 °C. Beads were centrifuged and rinsed with CHAPS buffer 5 times. The beads were resuspended in 100 μL of 50 mM Tris-HCl ...
The following antibodies were used in the study: anti-ApoM (ab66379, Abcam); anti-YAP (14074, Cell Signaling Technology); PCNA (13110, Cell Signaling Technology), anti-N-cadherin (13116, Cell Signaling Technology), anti-E-cadherin (ab11512, Abcam); anti-vimentin (5741, Cell Signaling ...
Immunoblotting was performed using standard SDS-PAGE protocols. YAP (#14074), p-S127 (#13008), ROCK1 (#4035), LATS1 (#3477), p-T1079 (#8654), p-S909 (#9157), MYPT1 (#5143), p-T853 (#4563) and GAPDH (#5174) antibodies were obtained from Cell Signaling Technology....
#14074 Filter: WB IP IHC IF F ChIP C&R Western blot analysis of extracts from control HeLa cells (Lane 1) or YAP knockout HeLa cells (Lane 2) using YAP (D8H1X) XP® Rabbit mAb (upper) or β-actin (13E5) Rabbit mAb #4970 (lower). The absence of signal in the YAP knockou...