The One Piece Wiki considers Yamato to be male. Yamato's gender identity is a topic of much debate, as there is evidence for both sides. Male pronouns are used by the official English translations from Viz and Crunchyroll/Funimation. It's important to note that despite rumors, the series ...
One of One Piece's biggest controversies is Yamato's gender identity, but the series' newest chapter should put the debate to rest.