(1:600, Abcam, ab16030), a PTP1B antibody (1:333, Abcam, ab52650), Bip (1:1,000, Cell Signaling Technology, 3177), eif2α (1:1,000, Cell Signaling Technology, 3398), XBP1 (1:200, Santa Cruz Biotechnology, SC-7160), and anti-β-actin antibodies (1:10,000, beta Actin ...
Chromatin was then incubated overnight with precoated protein G-Sepharose and anti-ATF6 (IMG-273, Imgenex), anti-XBP1 (sc7160, Santa Cruz Biotechnology), control IgG antibodies (3 μg), or no antibody. After washing, the immunoprecipitates were eluted two times with 200 μl of elution ...
Sigma-Aldrich), anti-phospho-eIF2α (#9721, Cell Signaling Technology), anti-eIF2α (#9722, Cell Signaling Technology), anti-IRE1α (#3294, Cell Signaling Technology), anti-sXBP1 (sc- 7160, Santa Cruz Biotechnology), and anti-Lamin A/C (sc-6215, Santa Cruz Biotechnology). An...
Chromatin extracts from lysed cells were sonicated to an average DNA fragment length of 350 bp and immunoprecipitated with Santa Cruz Biotechnology antibodies anti-XBP1 (sc-7160) or IgG isotype control (sc-2027). Following de-crosslinking, quantitative PCR was performed with multiple ChIP primers ...
(SC-6246), anti-Chk1 (SC-8408), anti-Cyclin B1 (SC-245), anti-Cyclin D1 (SC-246) and anti-XBP1 (SC-7160) for detecting XBP1s in animal model were from Santa Cruz Biotechnology (Dallas, TX, USA); anti-H2AX (ab124781) and anti-XBP1 (ab37152) for detecting XBP1u in the ...
Antibodies against XBP1 (sc-7160), HDAC3 (sc-136290) phospho-Akt (sc-7985R), Akt1 (sc-1619), Nrf2 (sc-722), mTOR (sc-1549), histone H3 (sc-10809), IRE1α (sc-20790), and GAPDH (sc-25778) were purchased from Santa Cruz Biotechnology; antibodies against FLAG (F2426, F1804, ...
The following antibodies were used (1:1000 dilution unless otherwise indicated): CHOP (sc-575) and XBP1 (sc-7160) (Santa Cruz Biotechnology, Santa Cruz, CA, USA); phospho-c-JUN (9164), phospho-JNK (Thr183/Tyr185, 9251), and total JNK (9252) (Cell Signalling Technology, Danvers, MA,...
1000), phospho-eIF2α (9721L; 1:1000), eIF2α (9722S; 1:1000), BiP (3183; 1:1000), and P58IPK (2940; 1:1000) were from Cell Signaling; AR (sc-816; 1:500), XBP1 (sc-7160; 1:500), ATF6α (sc-166659; 1:500), and GAPDH (sc-47724; 1:2000) were from Santa Cruz;...