将CreERT2-WPRE-pA插入到小鼠Wt1基因起始密码子处。 应用领域:Wt1在成年小鼠心脏的外膜表达,也在内脏器官的间皮中表达,可用作谱系追踪和标记Wt1表达细胞,常用于研究心脏、精子的发育及肝损伤等。 *使用本品系发表的文献需注明: Wt1-CreERT2 mice (Cat. NO. NM-KI-200127) were purchased from Shanghai Model...
总体来讲就是诱导性的特异性重组酶荧光小鼠：其中WT1：在肾脏和睾丸支持细胞中表达；ERT2：药物诱导；就是可以运用药物诱导前面WT1表达的Cre；Rosa26mTmG/+：就是一种红绿荧光报告系统，正常情况下在细胞膜表达tomato红色荧光，在有cre重组的情况下，会变成绿色GFP荧光；综上，就是在药物诱导下，WT1特异...
Wt1-CreERT2 品系全名 目录号 品系状态 C57BL/6Smoc-Wt1em1(CreERT2-WPRE-pA)Smoc NM-KI-200127 活体 基因信息 基因名 Wt1 基因曾用名 染色体位置 NCBI ID MGI ID Ensembl ID 人类同源基因 Wt-1; D630046I19Rik 22431 98968 ENSMUSG00000016458 WT1 品系描述 A CreERT2-WPRE-pA expression cassette was ...
ROSA纯合与带有CRE重组酶（一般是在特殊启动子下）的小鼠杂交,产生的F1代可以被表达的CRE切掉阻碍LACZ翻译的终止子,从而起始LACZ表达.因此某些特殊的组织或细胞就可以被LACZ染色追踪.
ERT2：超培部单药物诱导；就是可以运用360问答药物诱导前面WT1表达的Cre；Rosa26mTmG/+：就是一种红绿...
Comparison of the expression pattern of Wt1 and Cre in the healthy and infarcted heart.Bram van WijkQuinn D. GunstAntoon F. M. MoormanMaurice J. B. van den Hoff
人足细胞在高糖有或无时均表达WT1,而且WT1可以与另一个转录因子CRE结合蛋白(CBP)相互作用,而这种联系在25mM高糖存在时降低40%。对25mM高糖培养下人足细胞染色质进行染色体免疫沉淀法,发现WT1结合PCX起始序列能力降低部分可能因为WT1-CBP相互作用的损伤。将调节CBP和WT1联系的区域进行显性负性结构检测,发现WT1调节基因...
Using aWt1-/flox;Amh-Cre mouse model by deletingWt1in Sertoli cells (Wt1SC-cKO) at embryonic day 14.5 (E14.5),Wt1was found to regulate PMC and FLC development.Wt1deletion in fetal testis Sertoli cells caused aberrant differentiation and proliferation of PMCs, FLCs and interstitial progenitor ...
Similar results were obtained with Wt1CreERT2/Wt1flox/floxorgan cultures after tamoxifen-induced deletion of Wt1 and following FGF treatment (Supplementary Fig. 7D). Thus, irrespective of the gene-silencing method used, addition of exogenous FGFs rescues WT1-deficient kidney explants from apoptosis. ...
Pu (Boston, USA) for the Wt1 knockout, Wt1flox and Wt1CreERT2mice, respectively. We are grateful to Filippo Massa (iBV) for help with revisions and K. LeBrigand and P. Barbry (IPMC, Sophia Antipolis, France) and Elena Zotenko (Sydney, Australia) and the GENOMATIX team for initial ...