phytoalexininArabidopsis thaliana.InsearchofthetranscriptionfactorsdownstreamofMPK3/MPK6,wefoundthatWRKY33isrequiredforMPK3/ MPK6-inducedcamalexinbiosynthesis.Inwrky33mutants,bothgain-of-functionMPK3/MPK6-andpathogen-induced camalexinproductionarecompromised,whichisassociatedwiththelossofcamalexinbiosyntheticgene...
As gene structure is a typical imprint of evolution within a gene family, we analyzed theWRKYgenes inA. sinensisusing tools at the GSDS website. A detailed illustration of the gene structures is shown in Fig.2B. The number of exons ranged from 1 to 10, and more than half ofAsWRKYshave ...
The diversity of gene structure promotes the evolution of the gene family. To further understand the evolutionary process of the CtWRKY gene family, the structure and conserved motifs of theCtWRKYgene were analysed in combination with the CtWRKY protein phylogenetic tree (Fig.3). Ten conserved mo...
The first two reports were of ill-defined DNA binding proteins that played potential roles in the regulation of gene expression by sucrose (SPF1) [1] or during germination (ABF1 and ABF2) [2]. A third report identified WRKY1, WRKY2 and WRKY3 from parsley (Petroselinum crispum) and ...
The WRKY transcription factor (TF) gene family expanded greatly in the evolutionary process from green algae to flowering plants through whole genome, segmental, and tandem duplications. Genomic sequences from diverse plant species provide valuable infor
Xu et al. [17] identified a cotton GaWRKY1 gene containing a leucine zipper motif and provided strong evidences on the role of this TF in the regulation of CAD1-A gene, involved in the biosynthesis of the sesquiterpene gossypol by means of interaction with the W-box present in the CAD1...
Thus, our study revealed a unique dual function of IlWRKY22, an excellent candidate gene for breeding novel Iris cultivars of desirable traits. Keywords: Iris; WRKY transcription factor; flowering time regulation; salt stress; drought stress
Full size image Gene-chip based expression analysis of 26VvWRKYsunder various stresses Although we identifiedWRKYtranscription factors from theV. viniferagenome, functions for these genes in response to abiotic and biotic stress remain unknown. Using microarray results from publically available data, it...
Full size image Discussion The WRKY transcription factor genes are involved in the regulation of a large number of processes in plants. Previous studies have revealed some information on the WRKY gene family in many species, such asArabidopsis, rice, tomato, cotton, pineapple and wild strawberry....
RESULTS Identification and annotation of WRKY genes in the grapevine genome A total of 59 full-length genes encoding putative WRKY proteins were identified in the grapevine genome. Among them, 58 gene sequences were obtained from the 12X V1 prediction of the PN40024 grape genome sequence which...