The significant reduction in skin flora persisted to the time of wound closure, with culture positivity rates of 5.1% in the chlorhexidine group and 14.1% in the povidone iodine group.8 It is also prudent to establish a sterile field with the use of towels and/or drapes to safeguard against...
When surgical dressing kits are used for the provision of surgical dressings, all components of the kit will be considered not medically necessary. A surgical dressing kit is defined as non-individualized, standardized packaging containing repetitive quantities of dressings not related to the individual ...
The CM production is performed in the lab-scale quantities and based on cell culturing under predetermined conditions using supplemented cell culture media, which may contain additional components to promote cell polarization or growth factors [53,60,61]. Prior to the secretome harvesting, an antibiot...
making them an indispensable addition to any first aid kit. **Easy to Use and Portable** These iodine-disinfected cotton swabs are not just about effectiveness; they are also designed with convenience in mind. The swabs are easy to use, making them a go-to solution for smaller wounds such...
The cells on the upper surface of the filter membranes were gently removed by a wet swab after 24 hours of incubation. Then, 0.5% crystal violet (Beyotime, C0121) was applied to stain the filter membrane for 5 min. To evaluate their migration activity, the stained cells remaining on the ...
The well plates were placed in a cell culture incubator (37 °C, 5 % CO2) for 12 h to allow the cells to attach to the wall, and then the liquid was changed. KM hydrogel with 0.22 μm microporous filter was used for filtration and sterilization, and the media solution with...
Briefly, 1×105 cells were seeded in the upper chamber, while the lower chamber contained culture medium with 1% FBS and different concentrations of ASA VI (0, 20, 40 and 80 μg/mL). Cells treated with bFGF (50 ng/mL) were used as a positive control. After 12 h of incubation, the...
The cervical swab was used for isolation of Neisseria gonorrhoeae by culture and for detection of Chlamydia trachomatis antigen by Chlamydia microplate enzyme immunoassay kit (BIORAD). All data were analyzed using appropriate statistical tests. Results: All 40 cases were evaluated for the presence of...
DMEM high glucose culture medium (8121326, Biosharp, Beijing, China), HUVECs (HUM-iCell-e005, iCell, Shanghai, China), endothelial cell medium (PriMed-iCELL-002, iCell, Shanghai, China), glucose (G8769, Sigma, St. Louis, Missouri, USA), SOD Assay Kit WST (S0101S, Beyotime, Shang...
Cells remaining in the culture dish were counted as Nt. Cell seeding efficiency = (N0 - Nt)/ N0× 100%. Following cultivation in vitro for 1 to 7 days, nanofiber cytotoxicity was assessed using a live-dead cell staining kit based on provided directions, and imaged via fluorescence confocal...