Additionally, these materials are susceptible to in situ gel formation by folding and self-assembling process stimulated by cell culture media. The resultant hydrogels generally offer considerable advantages over polymeric hydrogels in terms of biodegradability and cytocompatibility in various mammalian cells,...
post-operative bleeding, and that the patients report less pain. Primary end-points of the trial are the maximum post-operative pain within 48 hours and the incidence of severe post-operative bleeding that requires surgical revision within 7 days after the surgical procedure. Secondary end-poi...
Cell culture Human embryonic skin fibroblasts CCC-ESF-1 (ESF) (generation 10, G10), human embryonic lung fibroblast CCC-HPF-1 (HPF) (G10), human immortal keratinocyte line (HaCaT) (G8), and human Umbilical Vein Endothelial Cells (HUVECs) (G3) were obtained from the National Infrastructur...
After 14 days of 3D culture, cell viability was verified by calcein-AM staining, and stemness surface markers were tested by flow cytometry (Fig. 1). The probe calcein-AM permeates cell membranes in a non-fluorescent form, and then it is converted into green fluorescent derivative by the ...
Furthermore, the burn wound tissues were subjected to transplantation using mesenchymal stem cells (MSCs) and were also examined on days 0, 7, 14, and 28 following the transplantation procedure. On day 7, all the groups that underwent transplantation exhibited wound progression and scab formation....
The patch fabrication procedure shown in Fig. 1A is simple, cost-effective, rapid, and highly reproducible. The material can easily be formulated using off-the-shelf reagents (all reagents can be commercially procured) and is selectively photocrosslinkable, rendering it compatible with digital light...
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One colony of each culture was transferred to the Müller-Hinton broth at a concentration of 1.0 × 108 cfu/ml according to the McFarland standard followed by seeding the bacteria in 96-well plates (each well contained 100 µl of microbial stock). Quality control analysis was ...
Briefly, a 200-µL aliquot of 1:100 dilutions prepared from overnight LB culture of A. baumanni was added to the wells of 96-well microtiter plates in the presence of different sub-lethal concentrations of DCD-1L (2 × , 4 × , and 8 × MIC) and incubated at 37 ...
A.L.W synthesized hydrogel biomaterial, J.H.K performed cell culture, RiboTag IP, and IHC image analysis. R.R.E prepared single-nuclei analysis plots, A.C and L.G.R generated the RiboTag mESC line, T.J.D. derived the plan for, and supervised, the biomaterial synthesis. M.V.S. ...