(Method: Culture aerobic and/or anaerobic on selected media; cell culture followed by use of direct immunofluorescence, nucleic acid amplification and DNA probe assays [e.g., Gen-Probe] are available for identification of Neisseria gonorrhoeae and Chlamydia trachomatis.) Negative: no...
(7 days) after the operation; Type II: purulent secretion with cellulitis, osteomyelitis, mediastinitis, exposed steel wire, and positive bacterial culture within a few weeks (2–6 weeks) after the operation; Type III: chronic sinus and chronic osteomyelitis are formed within months/years (6 ...
superficial specimens usually are not accepted for anaerobic culture, the only evidence of anaerobic bacterial involvement in a wound infection may be visualization on the Gram-stained smear of the mixed Gram-negative and Gram-positive cocci and bacilli characteristic of infecti...
Human umbilical vein endothelial cells (HUVEC) were prepared at a concentration of 1 × 105 cells/ml in a complete culture medium. Each well was supplemented with 300 µl of the cell suspension, corresponding to approximately 3 × 104 cells, in conjunction with nanofiber membrane ...
The fitted linear curves before and after 48 h of dynamic testing in a cell culture medium with HSF cells overlapped, indicating that the proposed biosensor has a long working life and can meet the practical demands for wearable wound care without the need for frequent bandage changes. ...
procedure, using Soft K-Cot® or SoftBiopsy® or SoftBiopsy® +D (more focal smaller tip on an applicator). The Kylon® hooks retain the sample, and the cot or the tip of the SoftBiopsy® can be placed in the appropriate medium for culture, molecular testing, or anatomic ...
A total of 171 cases of wounds of various aetiology were examined and screened bacteriologically for evidence of infection by surface swab culture and quan... S Basak,SK Dutta,S Gupta,... - 《J Indian Med Assoc》 被引量: 35发表: 1992年 A new histological procedure for re-evaluation of...
In this formula, the “blank value” refers to DMEM enhanced with 10% fetal bovine serum. The “control value” is associated with the spectral data obtained from the cell culture medium, whereas the “measured value” corresponds to the readings from the medium co-cultured with PATA-based hy...
Afterwards, the dye was aspirated and the culture further washed with PBS. The extent of dye penetrance in cell culture was assessed using an inverted microscope (Nikon Eclipse TS100). Pictures were taken at 10× with a SPOT Insight Fire Wire Camera (Diagnostic Instruments, Inc.; Sterling ...
Bacterial infections of wounds impair healing and worsen scarring. We hypothesized that transcriptome analysis of wounds infected with Klebsiella pneumoniae (K.p.) or Pseudomonas aeruginosa (P.a.) would indicate host-responses associated with the worse h