验证数据展示 WB analysis of fetal human brain using 26744-1-AP fetal human brain tissue were subjected to SDS PAGE followed by western blot with 26744-1-AP (WNT3A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.View All Images (2)...
Purification The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. Specificity This antibody detects endogenous levels of Wnt3a and Wnt3. Alternative Names MGC119418 ;MGC119419 ;MGC119420 ;Protein Wnt 3a Precursor ;Protein Wnt-3a ;Wingless typ...
to be useful as an analytical reagent in embryogeny, a diagnostic for cancers such as cancer of the breast, etc., especially an anti-Wnt-3a monoclonal antibody which is useful not only for an immunoprecipitation method and a Western blot method but also for a tissue immunity staining method....
WNT3A antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:1,000~1:2...
Western blot analysis of extracts of various cell lines, using WNT3A antibody. Background: The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation ...
Applications:ChIP, CyTOF-ready, ICC, IHC, ICFlow, Simple Western, WB Customers Who Bought This Also Bought beta-Catenin Antibody [Unconjugated] AF1329 beta-Catenin Antibody [Unconjugated] AF1329 Contact Information Product PDFs PDF SDS PDF Datasheet ...
The abundance of the Wnt3a protein in Wnt3a-CM and L-CM was detected by Western blot analysis19. Wnt3a-CM was depleted of the Wnt3a protein by incubation with 4 μg/mL rabbit anti-Wnt3a antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA) at 4 °C overnight. The conditioned ...
Sections were incubated with primary antibody (1:200) overnight at 4 °C, followed by respective secondary antibodies conjugated to Alexa Fluor 555 or 488 (1:500; Invitrogen) for 1 h at room temperature. After rinsing in PBS, slides were mounted with ProLong Gold with DAPI (Invitrogen). ...
Western blotting, radiographic examination and histological analysis show that ASCs-exos improve the ability for fracture repair in the rat model of nonunion bone fracture healing. Moreover, our results further proved that ASCs-exos play a role in activating the Wnt3a/β-catenin signaling pathway...
Wntless (WLS), an evolutionarily conserved multi-pass transmembrane protein, is essential for secretion of Wnt proteins. Wnt-triggered signaling pathways control many crucial life events, whereas aberrant Wnt signaling is tightly associated with many hum