Effectiveness of controlled-fat diets, an annotated bibliography, 1965–1968: Printed May 1968, Corn Products Food Technology Institute, 343 Winter St., Waltham, Mass., 02154, 21 p., no charge - ScienceDirect 来自 Elsevier 喜欢 0 阅读量: 19 作者:...
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temperature. Enzyme activities were corrected using fluorescence quenching. Fluorescence was measured using a microplate reader (EnSpire 2300 Multilabel Reader, Perkin Elmer, Waltham, MA, USA) with 355-nm excitation and 460-nm emission filters. The activities were expressed as μmol d−1g−1soil...
Following extraction, the samples were centrifuged at 8700 rpm for 10 min using a Heraeus Megafuge 8 (Thermo Fisher Scientific, Waltham, MA, USA). Then, 2 mL of the supernatant was filtered through a Phenex 15 mm, 0.2 µm PTFE syringe filter (Phenomenex, Torrance, CA, USA). The ...
After resting for 4 min, 75 µL of sodium carbonate solution were added and the mixture rested for 2 h in the dark, at room temperature; the absorbance was registered at 750 nm (Multiskan Go microplate spectrophotometer, Thermo Fisher Scientific Inc., Waltham, MA, USA). Gallic acid was ...
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Amplifications were on a PTC-200 Peltier Thermal Cycler (MJ Research Inc., Waltham, MA, USA) with the following program: (1) denaturation at 94.9 °C for 2 min; (2) subsequent denaturing at 94 °C for 1 min; (3) annealing at 67 °C for 45 s, −0.5 °C per cycle [40,41]...
For cell differentiation analysis, 200 μL of cell suspension was added in a cytospin slide chamber (Shandon EZ Double Cytofunnel, Thermo Scientific, Waltham, MA, USA), spun at 800 rpm for 5 min in a Cytospin 4 (Thermo Scientific, USA) and stained with Stain Set Diff-Quik (Siemens ...