AWestern blots showing signals obtained from whole cell extracts of parental and Bax-deficient cells, as indicated. GAPDH served as loading control.BWestern blots showing signals obtained from whole cell extracts of HCT116 cells following transfection with siRNAs targeting human Bax. GAPDH served as ...
The Applied Biosystems 7500 real-time PCR instrument was used for amplification. Transcript levels were normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and presented as percentage-change relative to average levels in mock-treated controls. Knockdown data are available in Supplementary ...
The membrane was incubated with rabbit antibody FAK, phosphorylated FAK (Tyr397) and GAPDH (Cst,1:1000 dilution) overnight at 4 ◦C. Then, the goat anti-rabbit antibody labeled with horseradish peroxidase was used to detect the first anti-60 min in TBST. The second binding antibody was ...