Whole-transcriptome analysis with total RNA sequencing (RNA-Seq) detects coding plus multiple forms of noncoding RNA. Total RNA-Seq can accurately measure gene and transcript abundance, and identify known and novel features of the transcriptome. ...
Fast and cost-efficient workflow—curated for RNA sequencing Flexible—prepare RNA-seq libraries from a wide range of sample types and input quantities Powered by Adaptase™ technology—for adapter tailing and ligation to 1st strand DNA; does not require 2nd strand DNA and leads to stranded ...
This study provides a whole transcriptome RNA-Seq analysis of separated cell types of the multicellular green algaV. carterif.nagariensisto reveal cell type-specific components and functions. To this end, 246 million quality filtered reads were mapped to the genome and valid expression data were ...
Previous whole-transcriptome analysis by RNA-Seq required hundreds of thousands of cells or microgram amounts of RNA. A modification of the cDNA library preparation method now allows unbiased capture of the majority of genes expressed in a single blastom
We conducted whole transcriptome RNA-seq on a subset of non-diabetic, and diabetic peripheral subcutaneous adipose tissue samples which we have earlier used in our gene expression microarray study6. We have also prioritized transcription factors and protein kinases as likely upstream regulators of ...
RNA-Seq analysis to capture the transcriptome landscape of a single cell 热度: 计算机知识windows系统:开始--运行--命令大全0421050529第一期 热度: mRNA-Seqwhole-transcriptomeanalysisofa singlecell FuchouTang1,3,CatalinBarbacioru2,3,YangzhouWang2,EllenNordman2,ClarenceLee2,NanlanXu2, ...
There are two primary approaches for whole transcriptome NGS: RNA-Seq and targeted transcriptome. RNA-Seq is recommended when one or more of the following are key to your research efforts: You want to detect all cellular RNA: mRNA, miRNA, tRNA, etc. ...
1. SENSE Total-Seq与RiboCop rRNA Depletion Kit绑定出售, poly(A) 筛选包括在SENSE mRNA-Seq中 2. 仅适用于去除掉rRNA的RNA 3. 24种内嵌式barcodes (Ion Torrent); 96种外源barcodes (SOLiD)单独出售; 包含了适用于Illumina的96种外源barcodes。
Whole transcriptome analysis of Acinetobacter baumannii assessed by RNA-sequencing reveals different mRNA expression profiles in biofilm compared to planktonic ... S Rumbo-Feal,MJ Gómez,C Gayoso,... - 《Plos One》 被引量: 88发表: 2014年 Diversity in the Major Polysaccharide Antigen of Acinetobact...
Compare microarrays and RNA-seq for transcriptome profiling Microarray analysis Gene arrays have become a powerful approach for comparing complex sample RNA populations. Using array analysis, the expression profiles of normal and tumor tissues, treated and untreated cell cultur...