Whole-transcriptome analysis with total RNA sequencing (RNA-Seq) detects coding plus multiple forms of noncoding RNA. Total RNA-Seq can accurately measure gene and transcript abundance, and identify known and novel features of the transcriptome. ...
RNA Library Prep Kitsupports a wider range of total RNA inputs (from 10 ng to 1 µg) than thexGen RNA Library Prep Kit(from 100 ng to 1 µg), the latter offers a faster workflow with fewer steps. This RNA-seq workflow is compatible with upstream mRNA enrichment and rRNA depletion...
RNA-SeqCadmiumGene ontologyKEGGCadmium (Cd) is a non-essential metal with no known biological function and a broad range of toxic effects in biological systems. We used whole-transcriptome sequencing (RNA-seq) to study the effects of Cd2+ toxicity in zebrafish liver cells, ZFL. The results ...
Previous whole-transcriptome analysis by RNA-Seq required hundreds of thousands of cells or microgram amounts of RNA. A modification of the cDNA library preparation method now allows unbiased capture of the majority of genes expressed in a single blastom
We conducted whole transcriptome RNA-seq on a subset of non-diabetic, and diabetic peripheral subcutaneous adipose tissue samples which we have earlier used in our gene expression microarray study6. We have also prioritized transcription factors and protein kinases as likely upstream regulators of ...
This study constitutes the first transcriptome-wide RNA-Seq analysis of separated cell types ofV. carterifocusing on gene expression. The high degree of differential expression indicates a strong differentiation of cell types despite the fact thatV. carteridiverged relatively recently from its unicellular...
Is it true that I can save time using Illumina Targeted Amplicon RNA-Seq over real-time PCR? It actually depends. The number of samples and targets possible in a single Illumina targeted amplicon RNA-Seq run depends on the Illumina platform and chemistry. Assuming the desired number of samples...
RNAseq-based whole transcriptome analysis in jejunum of pre-weaned calves under different milk feeding regimesHarald HammonDoerte FrietenCaroline GerbertChrista Kuehn
1. SENSE Total-Seq与RiboCop rRNA Depletion Kit绑定出售, poly(A) 筛选包括在SENSE mRNA-Seq中 2. 仅适用于去除掉rRNA的RNA 3. 24种内嵌式barcodes (Ion Torrent); 96种外源barcodes (SOLiD)单独出售; 包含了适用于Illumina的96种外源barcodes。
16 In a different study, targeted RNAseq resolved ∼60% of rearrangements in cases in which only 1 partner gene was known according to cytogenetics, including rearrangements involving RUNX1, ETV6, PDGFRB, and KMT2A.17 In the same study, a number of novel fusions were detected as well (...