Eosinophil leucocytes are extracted from mammalian blood, e.g of a horse or human, by centrifuging the blood, optionally after initial sedimentation to remove the bulk of the erythrocytes, discarding the serum and buffy coat layer of the sediment and separating the eosinophils or an eosinophil-...
The meaning of WHOLE BLOOD is blood with all its components (as white and red blood cells, platelets, and plasma) intact that has been withdrawn from a donor into an anticoagulant solution.
Whole blood was diluted with autologous red blood cell concentrate and platelet-poor plasma, which were obtained via centrifugation, in specific ratios to attain a final sample with a predetermined platelet count and hematocrit. P-selectin exposure and GPIIbIIIa activation in unstimulated platelets and...
Apheresis apparatus for separating an intermediate density component from whole bloodAn apheresis apparatus for increasing the purity and yield of platelets separated from donated whole blood by centrifugation incorporates a centrifuge for separating whole blood into lower, intermediate, and higher density ...
Whole blood analysis improves accuracy, eliminates centrifugation, reduces response time, and conserves blood volume. Several hand-held, and over 20 portable or transportable whole blood instruments are now available. Criteria for instrument evaluation include test menus, point-of-care features, analysis...
When available, plasma fraction was harvested after 20 min centrifugation at 1700 xgof blood collected in BD Vacutainer CPT tubes (BD, #362761). Plasma samples were treated by solvent/detergent treatment with a final concentration of 1% (volume) Triton X-100 (Thermo Fisher Scientific, #28314...
Whole blood can be used directly for the isolation of target cells. In some cases, however, the isolation of a particular cell type can be more effective with PBMCs as starting material. PBMCs can be generated from whole blood by density gradient centrifugation or erythrocyte ...
Whole blood samples were collected and tested at enrolment using interferon-gamma release assay (IGRA) and the GBP5 assay. Results: A total of 470 participants were enrolled, and 232 and 238 patients were finally diagnosed with aTB and non-TB, respectively. The GBP5 protein levels of aTB...
They are limited to either diluted 11,12 or lysed blood13,14, and in some cases, density based centrifugation to reduce blood complexity and cell-cell interaction15–21. The microfluidic cell sorting technologies that can handle whole blood, in general fall into two categories: label free ...
ASRB participant PBMCs were extracted from whole blood by centrifugation in Lymphoprep (Vital Diagnostics). Genomic DNA (gDNA) was extracted from PBMCs using salt extraction and quantified by PicoGreen assay (Life Technologies). The integrity of gDNA was determined by agarose gel electrophoresis pri...