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One round of automated gap closure using our in house gapResolution tool [46] resulted in a final assembly with 2272 contigs with an N/L50 of 139/74.8 kb. Newbler assembled consensus EST sequence data was used to assess the completeness of the final assembly using alignment with 90% ...
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another round ofcentrifugationfor 20 min, the infranatant was collected and protein concentration was determined with the Protein Assay Bicinchoninate Kit (Nacalai Tesque, Kyoto, Japan). Lysates of equal volume were resolved by SDS-polyacrylamide gelelectrophoresis(PAGE) using a Mini-slab size...