One such example is provided by the presence of two regions within the Neh6 domain of Nrf2 (in mouse Nrf2, these are residues 329–339 and 363–379) that support turnover of the CNC-bZIP protein even when Keap1 is inactivated.24 It has also been found that glycogen synthase kinase-3 ...
Each of these subfamilies of modifiers uses their private E1, E2 and E3 enzymes to ensure that a unique modifier is specifi- cally transferred through the respective private E2 and E3 enzymes onto the correct target substrate8,9. This elaborate specificity allows separate regulation of conjugation...
In these last two decades , fluorescent proteins (FPs) have become highly valued imaging tools for cell biology, owing to their compatibility with living samples, their low levels of invasiveness and the possibility to specifically fuse them to a variety of proteins of interest. Remarkably, the r...
Oil spills on surface waters are characterized by the release of hydro- carbons in the form of a thin film, mainly constituted by light, water-soluble hydrocarbons4. Eventually, these compounds dissolve in the water column or remain trapped within immiscible oil components, which become emulsified...
The success and validity of a machine learning classifier are predicated on the correct definition of the problem at hand, which in this case encompasses the definition of positive and negative datasets. Both the positive and negative datasets consist of experimentally validated peptide sequences which...
cells. Finally, our results showed that TMZ/Simva/ASH treatment potentially depends on an increase of Bax expression in GBM cells. Our current investigation might open new avenues for a more effective treatment of GBM, but further investigations are required for a better identification of the ...
canonical initiation factors known from cap-dependent translation initiation, except eIF4E, are necessary for initiation complex formation on the EMCV IRES and that a previously identified ITAF, the Polypyrimidine-tract binding protein (PTB), enhances 48S complex formation on the correct AUG start site...
If correct the function of RFH would partially overlap with that of tmRNA, but it would not have the tmRNA feature of ensuring the addi- tion of a C-terminal tag, which is the substrate for a spe- cific protease that rapidly degrades the product. The co-occurrence of RFH and the ...
The correct replacement of the target gene by the KANMX4 cassette in each deletion strain was confirmed by PCR amplification. All the deletant strains have normal growth phenotype. mRNA preparation, cDNA synthesis and hybridization For each experiment, freshly grown yeast cells were inoculated into ...
Both are correct but also incorrect, specifically since the “other causes” are not insignificant! In reality, the processes are interdependent and it cannot be said that a given cause is responsible for 3% of aging, while another one for 33.3%. This undermines the arguments of those who ...