Answer to: Although penicillin G has two amide functional groups, one is much more reactive than the other. Which amide is more reactive and why?...
What bacteria are generally not susceptible to penicillin? What are the sources of contamination in lab or in environment of microorganism? What kind of cells has viruses that can infect them? What makes Mycobacterium particularly resistant to staining?
T47D, MDA-MB-231, and MDA-MB-453 cell lines were maintained in Roswell Park Memorial Institute 1640 (RPMI) medium and MCF-7 was maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum supplemented with penicillin–streptomycin at 37 °C, 5% CO2, and...
The cells were maintained in Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum, 50 U/ml penicillin, and 50 μg/ml streptomycin at 37 °C in humidified atmosphere with 5% CO2. Transient transfection and luciferase assays All transfection experiments were conducted using...
Cell Culture—MCF-7 cells, a breast cancer cell line without P-glycoprotein and multidrug-resistant protein-1 expression, were maintained in RPMI 1640 medium containing 10% fetal bovine serum (MediaTech CellGro, Herndon, VA), penicillin (100 units/ml), and streptomycin (0.1 μg/ml) (Invitroge...
A. Penicillin B. Vancomycin C. Tetracycline D. Bacitracin E. Chloramphenicol Antibiotics: Antibiotics are medications or drugs that are used to treat infections and diseases caused by bacteria. A broad-spectrum antibiotic is an ...
The biological function of penicillin stems primarily from the re-activity of which of the following groups? A. Sulfide B. Cyclic amide C. Carboxlyic Acid D. Acyclic Amide Which drug works by inhibiting the activation of plasminogen? ...
for 24 hours at a cellular concentration of 2×107 ml in 5 ml polyethylene serum tubes (Sarstedt) containing Medium 199 with 17 mM glucose, 12 mM NaHCO3, 1 mM L-glutamine, 125 μg gentamycin/ml, 100 units penicillin/ml and 100 μg streptomycin/ml. Following maturation at 24 hours the...
supplemented with 5% (V/V) FBS (Hyclone; Logan, Utah), 2 mM L-glutamine and 2% penicillin/streptomycin (Gibco BRL); or reconstituted in PBS and used at the indicated concentrations. The peptides were greater than 90% pure. The anti-fusion inhibitor, RFI-641 (Raznikov et al., 2001) ...
Promoters commonly used in recombinant microbial expression vectors include the β-lactamase (penicillinase) and lactose promoter system (Chang et al.,Nature275:615, 1978; and Goeddel et al.,Nature281:544, 1979), the tryptophan (trp) promoter system1Goeddel et al.,Nucl. Acids Res.8:4057, 19...