Annexin V identifies surface-exposed phosphatidylserine, and 7-AAD is retained in late apoptotic cells. Early apoptosis (Annexin V+/7-AAD−) was then analyzed using FACScan flow cytometer (FACSVerse, BD Biosciences, Franklin Lakes, NJ, USA) for a minimum of 10,000 events. 4.10. Caspases-9...