[23]. 简要地,在30分钟潜伏期以后,反应通过攫取结冰终止在液氮,并且细胞被存放了在−30°C。 在测量的阵营之时,超声波振动毁坏细胞膜。 然后,在乙缩醛化样品以后,阵营EIA成套工具(Amersham Int。 plc. Buckinghamshire,英国)用于测量细胞内阵营水平。 价值被表达了作为阵营水平的百分之海拔在每次指向[translate] ...
Western blot procedures used were as per the protocol provided by Transduction Laboratories, Lexington, KY, and the ECL detection kit (Amersham Pharmacia Biotech, Buckinghamshire, UK). When comparing whole cell extracts, aliquots containing equal total protein amounts in the range 30–40 μg (Bio...