Although considerable progress has been made recently, there are still many problems that cannot be ignored, such as the variations among batches of organoids, the methods to obtain cells suitable for trans- plantation from retinal organoids [3,4], the safety and long-term survival of ...
The retina-like structures are dissected and collected in suspension for long-term culture. The third category is the classic procedure reported by Sasai et al. [2,44]. Briefly, ESCs are dissociated into single cells, and quickly re-aggregated in gfCDM + KSR medium in low-cell-adhesion V-...