During the past several years, scientists around the world have used this method to clone animals. They’ve created about a half-dozen different species, including the famous first sheep, Dolly, along with cows, mice, goats and pigs. Experts say these cloned animals could offer a great deal...
For safety reasons, active BoNT/X was cloned from two different fragments, one spanning the heavy chain and another one spanning the BoNT/X LC with the activation loop of BoNT/C. This strategy allows for a more efficient and controllable activation of the toxin using Factor Xa, as described...
Compared to other types of cells in the body, telomeres in sperm cells are the longest. High telomerase activity was detected in spermatogonia and spermatocytes of embryonic and adult testes in mice and humans (Wright et al., 1996; Martín-Rivera et al., 1998; Pech et al., 2015). Telome...
” (MLK, Jr.) When I was seventh grade, my teacher wanted me to help a boy who was very shy in our class, and I made him feel better and welcomed by inviting him to talk with us, helping him to know what we are talking about, and introducing him to my friends. The first time...
The entire ~7-kb fragment was then cloned into the cargo plasmid, pRL277 [42]. The sequence of the inserted fragment was confirmed by DNA sequencing. Conjugal transfer of the resulting plasmid from Escherichia coli to Synechococcus 7335 was performed as previously described [25,38]. Transcon...
In each case, after NB4-1Y was revived from storage on nutrient agar plates, inoculum cultures were first grown to early stationary phase (approximately six generations; 48 to 72 h depending on the substrate) on 6:2 FTAB, 6:2 FTSA, OCT and MgSO4 prior to inoculating exper- imental ...