The appts. for separating blood into its different constituents consists of a centrifuge (4), a blood extraction pumps (2, 3) a collector (6) connected to the centrifuge, and detect (8, 9) to control the process. The process is set in operation, when one of the detectors determines ...
Each sample was immediately frozen in a liquid nitrogen container to minimize metabolite degradation. After thawing and centrifugation at 6,000 × g at 4 °C for 15 min, samples were stored in 1.5 mL centrifuge tubes at −80 °C for further analyses. The methods and procedures for ...
Fasting serum total testosterone increased pre- to post-training, but more so in subjects consuming WPC (p < 0.05), whereas serum 17β-estradiol remained unaltered. SQ estrogen receptor alpha (ERα) protein expression and hormone-sensitive lipase mRNA increased with training regardless of ...
Although the “three-tube method” is susceptible to false results, serial tubes may be useful because an initial bloody sample portion may be discarded immediately and the least bloodstained sample used for analysis (at the laboratory, red blood cell counts may be done on partial samples from ...
Laboratories receiving samples for vitamin B12 assay in serum separator tubes that have already been centrifuged should either re-centrifuge the tubes, or aliquot and re-centrifuge serum from the tubes prior to vitamin B12 assay.LowreyDepartment of Clinical Biochemistry...
After inverting the tubes thrice to allow the samples to mix, the samples were stored at 4 °C for 16 h. Post 16 h incubation, samples were spun at 1500× g for 30 min at 4 °C (5810R centrifuge, Eppendorf), and the supernatants were removed. To remove PEG residues, the pellets ...