The WPA1 gene, encoding a von Willebrand factor type A (vWA) domain protein, was located on chromosome arm 7DS and isolated by map-based cloning. The functionality of WPA1 was validated by multiple independent EMS-induced mutants and gene editing. Phylogenetic analysis ...
aTertiary structure of Pm13 predicted by AlphaFold v2.0.bMLKL_NTD domain predicted by AlphaFold v2.0; the MLKL_NTD domain consists of a four-helix bundle (4HB) structure.cSTK domain predicted by AlphaFold v2.0, indicating the basic secondary functional features. Red: MLKL_NTD domain; Magenta:...
aTertiary structure of Pm13 predicted by AlphaFold v2.0.bMLKL_NTD domain predicted by AlphaFold v2.0; the MLKL_NTD domain consists of a four-helix bundle (4HB) structure.cSTK domain predicted by AlphaFold v2.0, indicating the basic secondary functional features. Red: MLKL_NTD domain; Magenta:...
Therefore, the G4 gene, which encodes a tandem kinase-vWA domain protein, emerged as the most likely candidate for Pm57 among the 12 genes. Fig. 2: Pm57 candidate gene identification using EMS-induced mutants. a Candidate gene identification by MutRNA-Seq. RNA-Seq reads from the parental ...
It encodes a tandem kinase protein with putative kinase-pseudokinase domains followed by a von Willebrand factor A domain (WTK-vWA), being ortholog of Lr9 that mediates wheat leaf rust resistance. The resistance function of Pm57 is validated via independent mutants, gene silencing, and transgenic...
(MLKL) protein that contains an N-terminal MLKL_NTD domain and a C-terminal STK domain7, and Pm57 encodes an unusual TKP protein with putative kinase-pseudokinase domains followed by a von Willebrand factor A (vWA) domain (WTK6b-vWA)8, which sheds light on various plant resistance ...
domain (START), and von Willebrand factor A domain (vWA)20,29,36,50,51,52These KFPs have emerged as a new type of receptor involved in the innate immunity in the Triticeae species36.MLKL-K/Pm13encodes a KFP whose putative functional STK domain is fused in the C-terminus of a unique ...
Green regions indicate the vWA and Vwaint domains. Red and orange arrows represent the position of each amino acid substitution in the corresponding mutants. IL, Phenotypes of different TraesCS7D02G010200 mutants from the Jing411 and ND5181 backgrounds. Scale bar, 1 cm. MN, A heterozygous ...
Only one high-confidence geneTraesCS7D02G010200was annotated in this region, which encodes an unknown protein with a putative vWA domain. Quantitative reverse transcription PCR (qRT-PCR) analysis showed thatTraesCS7D02G010200was mainly expressed in the spike. Haplotype analysis of 655 wheat ...
The domain structure of Sr43 was thus clearly different from that of proteins encoded by the ~290 cloned plant resistance genes, which were largely (73%) extracellular or intracellular immune receptors (Supplementary Table1). To explore the unusual structure of Sr43 in more detail, we used the...