0.2 g of low molecular weight chitosan (50,000–190,000 Da; 80% N-deacetylation; Product 448,869; Sigma Aldrich, USA) was dissolved in 1% (v/v) acetic acid solution and left under continuous stirring for 30 min. An anionic salt, Sodium Tripolyphosphate (STPP) (Na5P3O10; HSN Code:...
In each test and for each accession, approximately 25 seeds were planted in compost in a growth chamber set at 25 °C (with a photoperiod of 18 h light/6 h dark and a relative humidity of 80%). At the two-leaf stage, 10–15 well developed seedlings were selected in each ...
For the investigation of plant signaling pathways, seeds were sown in 30 × 40 cm boxes (40 plants per box). In both cases, wheat was grown under greenhouse semi-controlled conditions (natural photoperiod supplemented with artificial light if needed, with 20 ◦C ± 5 according to the ...
Seeds of wheat accessions were obtained from the working collection of the Indian National Gene Bank (INGB) at the ICAR-National Bureau of Plant Genetic Resources (NBPGR), New Delhi. Passport data of wheat accessions are provided in Supplementary Table S1. The geo-referencing of wheat ...
The distance between rows was 0.25 m, and 30 seeds were planted in each row. Fertilizer and weed management was similar to that of wheat breeding [83]. 4.2. Morphology Observation The male sterile individuals of NWMS1 were clear and visible at the booting stage. Whole plants were ...
F1 and Plant Growing Conditions The F1 produced by crossing Indian as the female parent and Icaro as the male parent was self-pollinated, and 120 F2 seeds were collected. The F2 mapping population was grown at the plant growth facility, Washington State University, under controlled conditions ...