If the amplicon coordinates are not known, map genomic assays to the genome, or gene expression assays to the transcriptome. A web-based tool that can be used in verification of coordinates is the UCSC Genome Browser In Silico PCR utility at: genome.ucsc.edu/cgi-bin/hgPcr. It is also ...
Other molecular aberrations include chromosomal instability, long non-coding RNAs (lncRNAs), Sonic Hedgehog (Shh), as well as modifications in genome, exome, transcriptome, and proteome. Apart from surgical management, which is usually the gold standard approach to chordomas, genomic findings in ...
Prior to RNA-Seq, the main technology for RNA analysis and gene expression profiling was microarrays. The main limitation of this technology is that it can only detect predefined RNA sequences, providing a biased view of the transcriptome. RNA-Seq is not limited to known sequences and produces...
Finally, a transcriptome also contains gene expression profiles that underlie arguably all phenotypic features of the cell at the time or state when the cell is characterized. What else are transcriptomes and transcriptomic clusters telling us? Hierarchical organization of transcriptomically defined cell ...
The three main uses of ribosome profiling are categorizing translated mRNA areas, observing how nascent peptides are folded, and measuring the volume of specific proteins synthesized. Other uses include (1) defining translated sequences within the complex transcriptome, (3) mapping translation initiation...
diagnostics Review A Pound of Flesh: What Cachexia Is and What It Is Not Emanuele Berardi 1,2,† , Luca Madaro 3,† , Biliana Lozanoska-Ochser 3, Sergio Adamo 3 , Lieven Thorrez 1 , Marina Bouche 3,* and Dario Coletti 3,4 1 Department of Development and Re...
Tools to gain insight into cell transcriptome RNA sequencing (RNA-seq) is a technique that gives researchers important insight into the transcriptome of cells. This NGS approach can be used in a number of fields including cancer biomarker research and inherited disease research. This DECODED provides...
‘vascular asymmetric appendicular structure initiated at the shoot apical meristem’. This definition is applicable to all vascular plants, but does not hold true for bryophytes (mosses, liverworts, and hornworts) as they lack a well-defined system of vascular tissue [1]. In fact, the leaf-...
Adding complexity, our understanding of what ‘self’ is has evolved over recent years with the emerging view that ‘self’ may extend beyond the genome-encoded proteome to include peptides from the microbiome or endogenous viral elements, as well as cryptic peptides generated from apparently ...
His group has applied multiple functional genomic approaches (proteome, transcriptome, and roboticized syn- thetic genetic arrays) to the study of this problem in yeast. He has found that deletions in 15 ribosome-biogenesis factors give mutant cells that are abnormally small. Many of the genes ...