List three factors that can denature a protein/enzyme. Name two types of local secondary structure in protein. What are the proteins found in the body and their functions? What are the three types of proteins? Explain each one of them. ...
Create an account Ask a question Our experts can answer your tough homework and study questions. Ask a question Search AnswersLearn more about this topic: PCR: Steps Involved in Polymerase Chain Reaction from Chapter 54 / Lesson 12 477K Polymerase chain reaction (PCR) is a laboratory ...
This targeting can occur both in vitro and in vivo, facilitating an array of molecular biology and biochemistry applications. These applications include things like enzyme-linked immunosorbent assays (ELISAs), Western blotting, and cell surface labeling. Enzymatic vs chemical biotinylation There are ...
Catalysts are vital in many biological reactions. Without a catalyst (often an enzyme) many of the reactions humans need to survive would occur too slowly. The addition of protein catalysts speeds things up. Use a Catalyst A catalyst is a substance that can alter the rate of a chemical react...
What is the function of primers in PCR? a. raw material for new DNA b. attach new nucleotides to DNA strand c. attach to either side of target DNA d. denature, or separate, DNA How do you analyze a DNA digestion pattern when you have DNA migration in agarose gel?
How is the enzyme used in PCR different from others? What are the three things required for DNA replication? How does this compare to what is required for PCR? Can RNA be amplified by PCR? Explain. What are the steps of a PCR?
List the 6 things you need to load the DNA sample into the gel. What is the purpose of the loading buffer? (a) What are the different types of DNA polymerase in prokaryotes and eukaryotes? (b) What are their functions? How is the enzyme used in PCR different from others?