ELISA substrate ELISA substrate: TMB for HRP ELISA substrate: pNPP for ALP Kinetics Very fast, typically within 5 minutes More linear, signal plateau typically at 60 minutes Advantages Rapid kinetics, TMB substrate is less chemically hazardous than pNPP More linear activity – allows for readings at...
Human Phosphatidylserine (PS) ELISA KIT is used for in vitro quantitative detection of Phosphatidylserine (PS) content in serum, plasma or other related biological fluids . Detection principle: This kit uses double-antibody sandwich ELISA method. Use Phosphatidylserine (PS) antibody to coat the enzyme...
Human choline acetylase (CHAC) ELISA kit is used for in vitro quantitative detection of serum, plasma, tissue, cell supernatant and The amount of choline acetylase (CHAC) in the relevant fluid sample. Experimental principle: This kit uses the double-antibody sandwich method to determine the samp...
After washing 100 µl of TMB solution was added and incubated for 30 min at room temperature in the dark. 50 µl stop solution was added to each well and absorbance was measured. Statistical analysis All gene expression values were log2-transformed. Calculation of group mean differences ...
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更多“What is the purpose of the Indian Prime Minister's speech?A.To reveal the secret in the 19”相关的问题 第1题 ELISA检测中应用最广泛的底物是()。 A.OPD B.TMB C.p-NPP D.4-MUU E.4-MU 点击查看答案 第2题 ELISA中应用最早的底物是A、OPDB、TMBC、5-ASAD、ABTSE、p-NPP ELISA中...
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is Horseradish peroxidase (HRP), which is commonly used in colorimetric ELISAs. When TMB detection substrate is added to ELISA wells a blue colored precipitate forms. Western blotting with HRP is also performed, where immobilized antigen-antibody complexes appear as colored bands on the assay ...
你知道HRP除了在ELISA中催化TMB产生蓝色产物,还有哪些使用场景吗?它可以在Western、EMSA、Southern或Northern等检测时催化ECL类试剂(如ECL、BeyoECL等)产生化学发光。也可以在免疫组化、免疫细胞化学或Western blot检测时催化DAB产生棕色沉淀。酶标二抗,那个酶绝大多数就是HRP。