What are the steps in the PCR cycle and what is the purpose for each? What is the function of the primers in a PCR reaction? What starting materials are required for the basic process of PCR and what does each of them do? What is the role of the DNA primers in PCR?
What is the function of DNA ligase in DNA replication? Answer the following questions regarding PCR reactions: \\ a. What do dNTPs provide during DNA replication? How are they different from dNMPs?\\ b. Why is Taq DNA polymerase commonly used in PCR,...
Real-world applications of the “DNA photocopier”2-6 From diagnostics, research, and prenatal care to agriculture and forensics, PCR techniques are an essential element in the arsenal of today's scientists. PCR is most commonly used in diagnosing infections like influenza, COVID-19, Human Immuno...
What is RT-PCR?Paul S. Auerbach
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A web-based tool that can be used in verification of coordinates is the UCSC Genome Browser In Silico PCR utility at: genome.ucsc.edu/cgi-bin/hgPcr. It is also important to make sure that amplicons are all approximately the same size, and that primer ...
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Using real-time PCR to perform SNP genotyping Real-time PCR enables you to screen known SNPs. The benefits of real-time PCR are that it is easy, accurate, and can scale to high throughput. Another advantage of real-time PCR is that the bioinfo...
What’s the function of the repetition of “completely”?根据最高人民法院、最高人民检察院《关于办理侵犯公民个人信息刑事案件适用法律若干问题的解释》的规定，商业银行工作人员非法获取、出售或者提供行踪轨迹信息、通信内容、征信信息、财产信息（ ）条以上的，应当认定为刑法第二百五十三条之一规定的“情节严重”...
what is the function of reverse transcriptase? Reverse transcriptase Reverse transcriptase, also called RNA-directed DNA polymerase, an enzyme encoded from the genetic material of retroviruses that catalyzes the transcription of retrovirus RNA (ribonucleic acid) into DNA (deoxyribonucleic acid). This ...