What is the function of DNA polymerases? 31. are joined to replicating strands ofDNAubonds. If the sequence of nucleotides on the original DNA strand wasA-G-G-C-T-A,what would be the nucleotide sequence on the complementary strand of DN...
DNA Polymerases: DNA polymerases are a group of enzymes which catalyze the formation of phosphodiester bonds between phosphate groups and ribose sugars. They are thus essential for DNA replication. Polymersase Chain Reaction (PCR): The Tas DNA polymerase is widely used in PCR, and has been since...
What is the function of DNA ligase in DNA replication? Explain about DNA replication. Which of the following is not a direct consequence of the fact that DNA polymerase can only build onto the end of an existing nucleic acid? (a) DNA replication requires...
Considerable controversy now surrounds the interpretation of the known structures of the nucleic acid polymerases and the orientation of the template-primers relative to the poly- merase active sites. The initial polymerase structure (that of the Klenow fragment of Escherichia coli DNA polymerase I) ...
While DNA polymerases generally replicate DNA accurately, occasional errors can arise, occurring at a rate of approximately one error per 10^7 nucleotides. Some DNA polymerases possess proofreading capabilities that help correct these errors. When a mismatched base pair is detected, the polymerase ...
Escherichia coli is View Solution DNA of Escherichia coli is View Solution Knowledge Check In E. coli the lac operon gets switched on when Alactose is present and it binds to the repressor Brepressor binds to operator CRNA polymerase binds to the operator Dlactose is present and it binds...
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On the other hand, primers are not labeled because their main function is to anneal to a specific DNA sequence and extend new DNA strands, not to be detected themselves. 12 In various scientific assays, probes are used to confirm the presence or absence of a target sequence or to measure...
The antiparallel orientation of DNA strands in the double helix allows each strand to serve as a template for repair. This orientation means that the strands run in opposite directions (5' to 3' and 3' to 5'), which is crucial for the repair mechanisms to recognize...
Nicotinamide adenine dinucleotide (NAD+) is involved in multiple physiological activities, including cell metabolism, energy synthesis, DNA repair, and immune function. Its concentration in the body affects cell senescence, and a decrease in NAD+ concentration accelerates this process. Studies have demons...