the column flow rate is 1-3BV/h, and elute with deionized water and alcohol aqueous solution, the elution flow rate is 2-4BV/h , collect the eluate and concentrate under reduced pressure;
Place the spin column in a clean tube and centrifuge. This ensures that all liquid is removed from the silica membrane. Place the spin column in a clean tube. Add RNase-free water to elute RNA from the silica membrane. Centrifuge. The eluate will contain the purified RNA.Answer...
The chromatogram makes little sense toanylayman as the peaks provide no information on the identity of the mixture components nor anyknowledgeon the amount present.That is why they aren’t able to learn much from the results. If you want to master the art of interpreting a chromatogram, you...
gradients. They are subsequently stored or, more commonly, directly applied to the mass spectrometer [71,74]. MudPIT approaches commonly use electrospray ionization (ESI) mass spectrometers since the chromatography eluate can be directly “online” analyzed with a connected ESI mass spectrometer [75]...
3) Mix the raffinate with 2-5 times alumina, dry it, pass it through alumina column chromatography, elute with isopropanol or ethanol solution, collect the eluate, and concentrate the eluate under reduced pressure , dry; 4) Re-dissolve the dried extract with ethanol, filter or centrifuge, rec...