Lysates were centrifuged (16 000 g, 10 min, 4 °C) and supernatants were measured for protein content using the bicinchoninic acid assay (Thermo Pierce, Rockford, IL, USA) per manufacturer's instructions. Three individual protein extracts were pooled (33.3 μg from each lysate giving 100 μ...
Monakhova and Lachenmeier [12] report that the effects of HMF in humans are not completely clear up so far. Development an in House Validation for 5-Hydroxy-2-methyl-furfuraldehyde (HMF)Analysis in Fermented Beverages Produced from Honey, Cane Syrup and Corn Syrup by HPLC-UV AN Hussain Mota...
Rudd et al., characterized the total serum N-glycans from advanced breast cancer patients using HPLC coupled with MS. They proved that there was a distinct increase in the sialyl-Lewis X epitope (CESLEX). The sialyl-Lewis X epitope is reported to be a better indicator for breast cancer ...